home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
 
  HUM-MOLGEN -> Genetic News | search  
 

Pluripotency by Piggybac

 
  April, 3 2009 3:02
your information resource in human molecular genetics
 
     

Researchers have developed a method to create induced pluripotent stem cells (iPSCs) with an unmodified genome. The technique, published online in Nature Methods, creates cells with the potential to differentiate into almost any cell in the body without introducing unwanted viruses into the cell's genetic makeup.

iPSCs are generated by 'reprogramming' body cells to become pluripotent. However, the viruses typically used to deliver the reprogramming factors integrate into the cell's genome, and can cause unwanted changes. This presents problems, in particular for clinical applications.

Allan Bradley and colleagues show that fully pluripotent iPSCs with an unmodified genome can be generated in the mouse. As in recently published work from two other groups, they use the piggyBac transposon as a vehicle to deliver the reprogramming factors to the cells. piggyBac is a piece of DNA that can integrate into the genome, but can later be removed without a trace. Notably, Bradley and colleagues show for the first time that the reprogrammed cells from which piggyBac has been seamlessly removed are fully pluripotent, as they contribute to multiple tissues in chimeric mice.

In addition, the authors incorporated a selectable gene into their reprogramming piggyBac construct. The gene renders cells that harbour the construct unable to grow in FIAU, a toxic agent. This strategy allows easy identification of iPSCs with an unmodified genome since upon removal of the piggyBac, these cells can grow in the presence of FIAU. This strategy will be useful in the future for isolating patient-specific human iPS cells with unmodified genomes.

Author contact:

Allan Bradley (Wellcome Trust Sanger Institute, Hinxton, UK)
E-mail:  abradley@sanger.ac.uk

Abstract available online.

(C) Nature Methods press release.


Message posted by: Trevor M. D'Souza

print this article mail this article
Bookmark and Share this page (what is this?)

Social bookmarking allows users to save and categorise a personal collection of bookmarks and share them with others. This is different to using your own browser bookmarks which are available using the menus within your web browser.

Use the links below to share this article on the social bookmarking site of your choice.

Read more about social bookmarking at Wikipedia - Social Bookmarking

Latest News
Variants Associated with Pediatric Allergic Disorder

Mutations in PHF6 Found in T-Cell Leukemia

Genetic Risk Variant for Urinary Bladder Cancer

Antibody Has Therapeutic Effect on Mice with ALS

Regulating P53 Activity in Cancer Cells

Anti-RNA Therapy Counters Breast Cancer Spread

Mitochondrial DNA Diversity

The Power of RNA Sequencing

‘Pro-Ageing' Therapy for Cancer?

Niche Genetics Influence Leukaemia

Molecular Biology: Clinical Promise for RNA Interference

Chemoprevention Cocktail for Colon Cancer

more news ...

Generated by News Editor 2.0 by Kai Garlipp
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 © 1995-2017 HUM-MOLGEN. All rights reserved. Liability, Copyright and Imprint.