A method for measuring in vivo protein occupancy in the entire yeast genome is presented in a study published online in Nature Methods. The technique will provide new details about how genes are regulated.
The idea behind this method is simple: genomic DNA that is bound by proteins is shielded from DNase I, an enzyme that digests the DNA, leaving an intact DNA fragment. This DNA fragment is a footprint of the protein, corresponding in size to the bound region. The sequence of the footprint reveals the binding sequence of the protein and therefore often allows the identification of the regulatory proteins.
John Stamatoyannopoulos and colleagues scaled this approach to a genome-wide level by using a technique for massively parallel sequencing of the DNase I-cleaved genomic DNA. They systematically mapped all footprints across the yeast genome and analyzed them for new and known protein binding patterns. As a result the authors were able to draw an intricate map of regulatory proteins along the entire yeast DNA.
This technique can be adapted to any organism with a sequenced genome and will allow new insights into the fine-scale architecture of genome regulation.
John Stamatoyannopoulos (University of Washington, Seattle, WA, USA)
Abstract available online.
(C) Nature Methods press release.
Message posted by: Trevor M. D'Souza
Bookmark and Share this page (what is this?)
Social bookmarking allows users to save and categorise a personal collection of bookmarks and share them with others. This is different to using your own browser bookmarks which are available using the menus within your web browser.
Use the links below to share this article on the social bookmarking site of your choice.
Read more about social bookmarking at Wikipedia - Social Bookmarking