home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
  HUM-MOLGEN -> Genetic News | search  

Finding Footprints in the Genome

  April, 3 2009 3:00
your information resource in human molecular genetics
A method for measuring in vivo protein occupancy in the entire yeast genome is presented in a study published online in Nature Methods. The technique will provide new details about how genes are regulated.

The idea behind this method is simple: genomic DNA that is bound by proteins is shielded from DNase I, an enzyme that digests the DNA, leaving an intact DNA fragment. This DNA fragment is a footprint of the protein, corresponding in size to the bound region. The sequence of the footprint reveals the binding sequence of the protein and therefore often allows the identification of the regulatory proteins.

John Stamatoyannopoulos and colleagues scaled this approach to a genome-wide level by using a technique for massively parallel sequencing of the DNase I-cleaved genomic DNA. They systematically mapped all footprints across the yeast genome and analyzed them for new and known protein binding patterns. As a result the authors were able to draw an intricate map of regulatory proteins along the entire yeast DNA.

This technique can be adapted to any organism with a sequenced genome and will allow new insights into the fine-scale architecture of genome regulation.

Author contact:

John Stamatoyannopoulos (University of Washington, Seattle, WA, USA)
E-mail: jstam@u.washington.edu

Abstract available online.

(C) Nature Methods press release.

Message posted by: Trevor M. D'Souza

print this article mail this article
Latest News
Variants Associated with Pediatric Allergic Disorder

Mutations in PHF6 Found in T-Cell Leukemia

Genetic Risk Variant for Urinary Bladder Cancer

Antibody Has Therapeutic Effect on Mice with ALS

Regulating P53 Activity in Cancer Cells

Anti-RNA Therapy Counters Breast Cancer Spread

Mitochondrial DNA Diversity

The Power of RNA Sequencing

‘Pro-Ageing' Therapy for Cancer?

Niche Genetics Influence Leukaemia

Molecular Biology: Clinical Promise for RNA Interference

Chemoprevention Cocktail for Colon Cancer

more news ...

Generated by News Editor 2.0 by Kai Garlipp
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 © 1995-2023 HUM-MOLGEN. All rights reserved. Liability, Copyright and Imprint.