home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
 
  HUM-MOLGEN -> Genetic News | search  
 

A model for studying cell plasticity in the embryonic microenvironment

 
  March, 15 2006 6:19
your information resource in human molecular genetics
 
     
Reprogramming metastatic melanoma cells to assume a neural crest cell-like phenotype in an embryonic microenvironment
Kulesa, P.M., Kaselmeier-Kulesa, J.C., Teddy, J.M., Margaryan, N.V., Seftor, E.A., Seftor, R.E.B., and Hendrix, M.J.C. Proc. Nat. Acad. Sci. site, 103, 3752-3757 (March 7, 2006).

This work extends the study of tumor cell plasticity to a new model, the chick embryo, by demonstrating that the microenvironment alters phenotypic expression of transplanted human metastatic melanoma cells. Specifically, the results show that melanoma cells transplanted into 7- to 9-somite chick embryos do not form tumors, but rather, express phenotypic traits resembling those of neural crest cells (NCCs). After 48 hours, the cells migrate into the mesoderm layer and subsequently distribute along the cranial NCC migratory pathways, including the dorsal root and sympathetic ganglia. Moreover, they tend not to spread laterally, but follow the normal cells’ trajectories. Their migration appears to follow cues provided by the microenvironment, rather than the indigenous NCCs themselves, as ablation of the NCCs had no effect on melanoma cell distribution.

The transplanted melanoma cells exhibited morphological features closely resembling those of chick NCCs in the formation of filopodial connections between adjacent cells and linear chain-like arrays. However, differences were also observed, notably less hairy and bipolar shaped-morphologies. To determine whether the embryonic microenvironment also resulted in the expression of phenotype-specific genes, a study looked for several markers of melanocytes, neurons, and glia that the original metastatic melanoma cells did not exhibit. Small numbers of the transplanted cells expressed a melanocyte-specific marker (MART-1), while others tested positive for a neuronal marker (TuJ1).

Overall, the results indicate that metastatic melanoma cells transplanted into chick embryos create a model for studying signals that drive normal embryogenesis and factors that might prove useful in combating certain types of cancer.


Message posted by: Keith Markey

print this article mail this article
Bookmark and Share this page (what is this?)

Social bookmarking allows users to save and categorise a personal collection of bookmarks and share them with others. This is different to using your own browser bookmarks which are available using the menus within your web browser.

Use the links below to share this article on the social bookmarking site of your choice.

Read more about social bookmarking at Wikipedia - Social Bookmarking

Latest News
Variants Associated with Pediatric Allergic Disorder

Mutations in PHF6 Found in T-Cell Leukemia

Genetic Risk Variant for Urinary Bladder Cancer

Antibody Has Therapeutic Effect on Mice with ALS

Regulating P53 Activity in Cancer Cells

Anti-RNA Therapy Counters Breast Cancer Spread

Mitochondrial DNA Diversity

The Power of RNA Sequencing

‘Pro-Ageing' Therapy for Cancer?

Niche Genetics Influence Leukaemia

Molecular Biology: Clinical Promise for RNA Interference

Chemoprevention Cocktail for Colon Cancer

more news ...

Generated by News Editor 2.0 by Kai Garlipp
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 © 1995-2017 HUM-MOLGEN. All rights reserved. Liability, Copyright and Imprint.