HUM-MOLGEN archive: BIOT: ABI, fluorescent primers

Date sent: 21-SEP-1995 15:45:17 This message contains three posts relating to ABI fluorescent genotyping and primer supply. Cheers, Martin Kennedy/Arthur Bergen BIOT Topic Editors, HUM-MOLGEN 8< ======================= cut here ======================= >8 Date: Mon, 18 Sep 1995 13:50:13 GMT From: Philip J Asherson <WMGPJEA@CARDIFF.AC.UK> Subject: Re: BIOT: ABI Genotyping We have developed our own system for the rapid analysis of ABI genotype data for linkage studies. We are currently up to 15 loci per lane on a 36 well gel, running 3 gels per day. We have found that gel to gel variation can produce as much as +/- 2 bp chahnges in mobility on a 12 cm gel. We have developed our own software to overcome this. Philip Asherson Institute of Medical Genetics Cardiff 8< ======================= cut here ======================= >8 Date: Mon, 18 Sep 95 14:22:07 EDT From: Mark.Perlin@J.GP.CS.CMU.EDU Subject: Re: BIOT: ABI Genotyping Our group is developing software that automates the use of ABI (and other gel-based) genotyping data. The key innovation is the computer-based elimination of PCR stutter artifact (1,2) in poly-nucleotide repeat markers. - Mark ---------- Mark W. Perlin, Ph.D., M.D., Ph.D. Senior Research Scientist Computer Science Department Carnegie Mellon University Pittsburgh, PA 15213 perlin@cmu.edu (412) 521-7421 (412) 521-5204 (FAX) ---------- (1) Perlin, M.W., Burks, M.B., Hoop, R.C., and Hoffman, E.P. (1994). Toward fully automated genotyping: allele assignment, pedigree construction, phase determination, and recombination detection in Duchenne muscular dystrophy. Am. J. Hum. Genet., 55(4): 777-787. (2) Perlin, M.W., Lancia, G., and Ng, S.-K. (1995). Toward fully automated genotyping: genotyping microsatellite markers by deconvolution (GMBD). Am. J. Hum. Genet.: in press. 8< ======================= cut here ======================= >8 Date: Thu, 21 Sep 1995 09:08:14 +1410 From: Agnes Tay <mcbtayhn@LEONIS.NUS.SG> Subject: fluorescent primers supply? Hi My collaborators and I are embarking upon a long-term project to study the genetics of several neurological and neuropsychiatric disorders in Singapore. We have been promised access to ABI equipment and software which should greatly speed up everything. Our main concern is the cost of fluorescent primers and we'd like to know how to get reliable ones at minimal cost. One of our approaches involves the use of previously defined microsatellite markers. I've heard that these are sometimes available for free from some suppliers. Anybody knows how we can apply for these? Thanks very much, Agnes 8< ======================= cut here ======================= >8