Multifunctional Polymeric Nanoparticles from Diverse Bioactive Agents
Bertin, P.A., Gibbs, J.M., Shen, C.K.F., Thaxton, C.S., Russin, W.A., Mirkin, C.A., Nguyen, S.T. J. Amer. Chem. Soc.,128, 4168-4169 (April 5, 2006).
Scientists at Northwestern University have used a ring-opening metathesis polymerization method to create nanoparticles with a core, which may be used to carry drugs, and a shell surface that permits attachment of single-stranded DNA or tumor-targeting antibodies. The spherical structures were confirmed with transmission electron microscopy as having diameters less than 200 nm. Tosyl groups on the shell surface were used to attach single-stranded oligonucleotides via 5’-terminal amines and antibodies via amines on such amino acids as lysine. Transmission electron microscopy confirmed the attachment of the biologically active moieties. Moreover, when the nanoparticles were conjugated with both anti-HER-2 IgY and Bcl-2 antisense oligodeoxynucleotides, human breast carcinoma cells that overexpress the HER-2 protein internalized the nanoparticles. Exposure of the cells to Bcl-2 alone or to nanoparticles lacking the HER-2 antibodies showed no uptake. Hence, the HER-2 antibodies facilitate the entry of the nanoparticles into the cell.
The authors previously have shown that chemotherapeutic agents can be sequestered in their nanoparticles’ cores. Merging that approach with the one used in the present study should yield a wide range of anticancer therapies, delivered with specificity and with the potential for obtaining synergistic effects of different drug combinations.
Message posted by: Keith Markey
Bookmark and Share this page (what is this?)
Social bookmarking allows users to save and categorise a personal collection of bookmarks and share them with others. This is different to using your own browser bookmarks which are available using the menus within your web browser.
Use the links below to share this article on the social bookmarking site of your choice.
Read more about social bookmarking at Wikipedia - Social Bookmarking
Variants Associated with Pediatric Allergic Disorder
Mutations in PHF6 Found in T-Cell Leukemia
Genetic Risk Variant for Urinary Bladder Cancer
Antibody Has Therapeutic Effect on Mice with ALS
Regulating P53 Activity in Cancer Cells
Anti-RNA Therapy Counters Breast Cancer Spread
Mitochondrial DNA Diversity
The Power of RNA Sequencing
‘Pro-Ageing' Therapy for Cancer?
Niche Genetics Influence Leukaemia
Molecular Biology: Clinical Promise for RNA Interference
Chemoprevention Cocktail for Colon Cancer
more news ...