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A tale of a tail: to condense or not to condense....Ac is the question.

  March, 27 2006 17:05
your information resource in human molecular genetics
The N-terminal tail of histone proteins provides a platform for post-translational modifications that are important for gene activity. One such modification — the acetylation of histone H4 at Lys16 (H4K16Ac) — is associated with transcriptional activation and the maintenance of euchromatin. Craig Peterson and colleagues now report in Science that this single histone modification inhibits chromatin folding, and thereby keeps the chromatin in an 'open' configuration that allows gene transcription to take place.

Previous studies have shown that a region of the H4 tail containing residue K16 is important for the formation of 30-nm chromatin fibres. To test whether the modification of K16 might control higher-order chromatin formation, Peterson and colleagues chemically synthesized an H4-tail peptide that contained an acetylated K16 residue and attached it to the remaining part of the H4 protein. The recombinant H4 protein was incorporated into histone octamers, which were assembled with a DNA template into nucleosomal arrays.

Under conditions in which the nucleosomal arrays adopted a 'beads-on-a-string' conformation, arrays that contained either the wild-type histone H4, H4K16Ac or H4-N (which lacks the N-terminal tail region) were analysed, and all showed nearly identical sedimentation coefficients. However, by adding magnesium salt, which promotes chromatin compaction, the average sedimentation coefficient of the wild-type H4 arrays increased, which was consistent with the formation of compact, 30-nm-like fibres. By contrast, arrays that contained either H4K16Ac or H4-N were unable to condense fully and showed only a moderate increase in the average sedimentation coefficient.

A further increase in the magnesium-salt concentration promotes the self-association of the arrays through fibre–fibre interactions that stabilize higher-order chromatin domains. Self-association of the arrays that contained H4K16Ac or H4-N required higher concentrations of magnesium salt compared with those comprising wild-type H4. So, the acetylation of H4K16 had the same effect as deleting the entire H4 tail and inhibits 30-nm fibre formation as well as cross-fibre interactions. Indeed, H4K16Ac was associated with decondensed chromatin in vivo, as nucleosomal arrays that contained H4K16Ac were present predominantly in MgCl2-soluble chromatin fractions, which are known to be enriched in transcriptionally active genes.

Finally, the Peterson team tested whether the H4K16Ac modification affects the function of the ATP-dependent chromatin assembly and remodelling factor (ACF) complex, which associates with the H4 tail to enable nucleosomes to slide along the DNA. They showed that wild-type mononucleosomes that had been incubated with ACF exhibited significant sliding, whereas sliding by H4K16Ac-containing nucleosomes was reduced.

These results show that a single histone modification can dramatically change the state of chromatin by inhibiting the formation of higher-order chromatin and regulating the activity of a chromatin remodelling enzyme, thereby keeping the chromatin in a decondensed, transcriptionally active conformation

Message posted by: Simon Chandler

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