HUM-MOLGEN archive: BIOT: for March

New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 5500 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! - Please send high quality messages only, including full name, address and purpose. - Please use the appropriate TOPIC subject heading in your message. - Please state non-trivial questions only. - Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community. - Messages may be refused without further notification. Edward Wilcox Trevor M. D'Souza (BIOT editors) *********************************************************** This BIOT message contains: [1] How can I show DNA-protein interaction? [2] Where can I find basic information about the BRAC1/2 gene? [3] Information about NetPrimer: Free Primer Analysis on the Internet. [4] I need information on genetic engineering for a high school research paper. [5] Where can I get scientific literature on the role of genetic engineering in sports science? [6] I am looking for information on Zeolite coupled with oxides used as an inorganic catalyst for medical purposes. [7] Is there any improved method for in situ hybridization? [8] I need information for a research paper on germline or designer babies. ************************************************************** [#1] Hi, How can I identify whether a protein is able to interact with DNA? Dr Liu YP Beijing Biotechnology Institute, Beijing China E-mail: l.yi@MAILCITY.COM ----------------------------------------------------------------------------- [#2] Hi: I am a 15-year old. I am writing a research paper of the technicalities of the actual testing for the BRAC1/2 genes. I have been reading a lot about it but everything is too hard to understand. Could you please send me the basics? Thanks Sara, Ithaca NY E-mail: slustick@TWCNY.RR.COM ----------------------------------------------------------------------------- [#3] Dear Editor, We would like to tell your membership of a new FREE resource on the Internet, NetPrimer, which we believe is the most comprehensive primer pair analysis program available on the Internet. Here is additional information: NetPrimer is a web-based program that analyzes individual or pairs of primers. It is available free of charge. The program combines the latest primer design algorithms with an intuitive interface allowing the user to quickly analyze primers. Primers are analyzed for melting temperature using the nearest neighbor thermodynamic theory to ensure accurate Tm prediction. Primers are analyzed for all secondary structures including hairpins, self-dimers, and cross-dimers in primer pairs. This analysis ensures the availability of the primer for the reaction as well as minimizing the formation of primer dimer. The program eases quantitation of primers by calculating primer molecular weight and optical activity. To facilitate the selection of an optimal primer, each primer is given a rating based on the stability of its secondary structures. Extensive help including algorithms and formulae used is available online. A complete analysis report can be printed for individual primers or primer pairs. Please take a look at NetPrimer by visiting: http://www.PremierBiosoft.com/NetPrimer.html Looking forward to a reply from you, Sincerely, Kay Brown PREMIER Biosoft International Developers of Intuitive Software for the Molecular Biologist 3786 Corina Way, Palo Alto, CA 94303-4504 Tel: 650-856-2703; Fax: 650-843-1250 Web: http://www.PremierBiosoft.com E-mail: kbrown@PremierBiosoft.com ----------------------------------------------------------------------------- [#4] Dear Sir: I need general information on genetic engineering for a research paper. Could you please send me some or give me an address where I can write for some? Thanks, Jill Lemoine E-mail: JLEMO64164@AOL.COM ----------------------------------------------------------------------------- [#5] Dear Sirs/Madams: My name is Christian Di Zio and I am doing my honours degree at the University of the Witwatersrand in Gauteng, South Africa. I have recently become very interested in the field of human biotechnology/genetic engineering. I was lucky enough to be chosen to do my theoretical project on the subject of: "Does genetic engineering have a justifiable role to play in sports science?" One month after an intensive literary research it has become apparent to me that very little has been speculated on the subject! This leaves me with quite a big problem. My report is due in two weeks and I have really battled to find a substantial amount of literature. I would be eternally indebted to you if you could please reply to this mail with any information or opinions you have on this subject. Any type of reply; e.g. Internet sites, journal articles (or their references), opinions on the ethical or physiological role of genetic engineering in sports science, or even other e-mail addresses to which I can apply to; would suffice. Your help is greatly appreciated. Faithfully Christian Di Zio Department of Physiology University of the Witwatersrand E-mail: christian_di_zio@hotmail.com OR phslcri@chiron.wits.ac.za ----------------------------------------------------------------------------- [#6] Hi, My name is Syed Ameeruddin. I am looking for information on Zeolite coupled with Pt or other oxide used as an inorganic catalyst for medical purposes. My email address is: surgeon80@hotmail.com ----------------------------------------------------------------------------- [#7] Is there any improved method for in situ hybridization using very small (20-100bp) sequences inserted in plasmid for (FISH)? Bharati Hukku E-mail: bhukku@yahoo.com ----------------------------------------------------------------------------- [#8] I am writing a research paper on germline or designer babies and I am hoping for some helpful information. Please get in touch with any additional information. Thanks, Erika Reimerdes E-mail: ereimerd@bu.edu **************************************************