HUM-MOLGEN archive: BIOT: March-April 1998

New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 5016 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! -Please send high quality messages only, including full name, address and purpose. -Please use the appropriate TOPIC subject heading in your message. -Please state non-trivial questions only. -Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Trevor M. D'Souza (BIOT editors) This BIOT message contains: 1) A request for information on constructing full length cDNA libraries 2) Do you know where we may get a chromosome 17 cosmid library? 3) A search for sheep probes, cell lines and information 4) Help in PCR amplification 5) A search for software to estimate similarities to known DNA sequences 6) Does anyone have a suggestion of a small laboratory animal whose growth plate closes with sexual maturity? ***************************************************************************** We are working on genome analysis in pigs, and we are going to make a cDNA library. Since we want expression of a specific gene (we don't know the size or anything about nucleotide composition; positional cloning), we need as many full length cDNAs as possible. Does anybody know a simple, but efficient way of enriching for full lengths? References to papers would be nice too. Best regards, and thank you Trine Winteroe akw@kvl.dk Department of Breeding and Genetics The Royal Veterinary and Agricultural University Groennegaardsvej 3 1870 Frederiksberg C, Denmark ***************************************************************************** Dear colleagues: Do you know where we may get specific chromosome cosmid library (i.e. Chromosome 17)? Or what would be the most efficient approach to map a newly cloned gene? Your assistance or comments would be greatly appreciated. Sidong Fu, M.D. sfu@UMARYLAND.EDU University of Maryland Tel: 410-706-4225 (lab) Fax: 410-706-0020 ***************************************************************************** Dear Colleagues At the moment we are working on UV-induced squamous cell carcinoma in humans and sheep. We are looking for information on: sheep genetics sheep cell lines sheep DNA probes. Does anyone has any information about these subjects. It would be appreciated very much. Dr. W. Kalle wkalle@csu.EDU.AU Lecturer in Molecular Biology School of Biomedical Sciences Charles sturt University P.O. Box 588 Wagga Wagga NSW 2678 Australia phone 069-332409 fax 069-332587 Schools home-page: http://www.csu.edu.au/faculty/health/biomed/ ***************************************************************************** I'm trying to amplify a region of DNA. I was getting PCR product but it included many different sized fragments. I thought it might be my primers so I did a PCR with only one of each of my primers. With each primer individually, I got product! My region is G+C - rich (about 75%) so I used 7-deaza-dGTP (ratio of 3/1 with dGTP). Are my primers amplifying regions that can self-prime? Does this have anything to do with the high G+C? Should I design new primers or is there something I can do to fix my product? Thank you very much, Shannon Bradley s_bradl@hartco.ca (Shannon Bradley ) McGill University Department of Human Genetics 1033 Pine Avenue Montreal, Quebec CANADA ***************************************************************************** Dear Colleagues I am trying to find out software programs either on the internet or PC based that can be used for searching for similarities to known DNA sequences and to determine homology if any. Would be most grateful if you could help me out here. Thanks E. Cline Ph.D. ecline@gpu.srv.ualberta.ca ***************************************************************************** Having studied aspects of pathology of the femoral head of rats, we find the fact that these animals' growth plates remain open throughout life, disturbing. Does anyone have a suggestion of a small laboratory animal whose growth plate closes with sexual maturity? Thanks in advance for your help. jhboss@netvision.net.il (J. H. Boss) *****************************************************************************