HUM-MOLGEN archive: BIOT: various July-August 1997

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Edward Wilcox Arthur Bergen (BIOT editors) This BIOT message contains: 1) RT-PCR GADP pseudogene 2) Methylation-sensitive RDA 3) Collaboration asked for K+ channels on chromosome 3 4) Telomerase inhibitors in vivo 5) LDL binding assays 6) endometrial premalignant lesions as well as frank endometrial tumors asked 7) antibody against human Facc protein wanted ********************** This message was originally submitted by michael_kern@SMTPGW.MUSC.EDU to the HUM-MOLGEN list at NIC.SURFNET.NL. MISC: GAPDH pseudogene? We are performing RT-PCR on some human tissue samples and have made primers to GAPDH as a positive control. These primers are from the first and fith exons, therefore they should generate about a 320 bp band when amplifying fully processed mRNA (cDNA) or a 2.2 Kb band when amplifying genomic DNA. However, when we perform PCR on human genomic DNA we obtain a 320 bp band. We believe we have eliminated the possibility that the DNA is contaminated by RNA or the possibility that the PCR reaction (reagents) is contaminated. At this time we believe the only other explanation is that the human genome has a pseudogene for GAPDH. We have checked the Genbank databases and find no evidence for a GAPDH pseudogene. Has anyone else ever experienced this same (or similar) problem? Is there any evidence for a GAPDH pseudogene? Thank you for any comments/suggestions. Michael J. Kern, Ph.D. E-mail: kernmj@musc.edu ****************************************** Reply-To: "C.S.Kua" <cskua@mailhost.unimas.my> Subject: Help wanted (mailing list): MS-RDA cskua@mailhost.unimas.my (C.S.Kua) sent the following comments: ------------------------------------------------------------ Dear everyone, I am interested to find out more about methylation sensitive RDA. Any information would be much appreciated. Thank you very much. Yours sincerely, C.S.Kua Institute of Health & Community Medicine Universiti Malaysia Sarawak 94300 Kota Samarahan Sarawak Malaysia Fax : 6-082-671903 Tel : 6-082-671000 ext282 e-mail : cskua@mailhost.unimas.my *********************************** Reply-To: Ali Riazi/H McDermid <ariazi@gpu.srv.ualberta.ca> ariazi@gpu.srv.ualberta.ca (Ali Riazi/H McDermid) sent the following comments: ------------------------------------------------------------ Dear Colleagues, We have a gene with high similarity to subunits of Ca+2-activated potassium channels on chromosome 3. Would someone be interested to collaborate with us on further mapping and characterization of this gene. Thanks Ali Riazi/ H. McDermid Department of Biological Sciences University of Alberta -ariazi@gpu.srv.ualberta.ca ****************************** Reply-To: Sabine Swierenga <sabine@unixg.ubc.ca> From: Sabine Swierenga <sabine@unixg.ubc.ca> Subject: telomerase sabine@unixg.ubc.ca (Sabine Swierenga) sent the following comments: ------------------------------------------------------------ Is anyone aware of any in vivo work with telomerase inhibitors, animal of clinical? ---------------------------------------------------------- **************************** From: Chris Friedrich <cfriedri@MAIL.MED.UPENN.EDU> Subject: Re: DIAG:LDL receptor assays X-To: Human Molecular Genetics Are any laboratories currently doing LDL binding activity assays on cultured fibroblasts or amniocytes? Chris Friedrich, M.D., Ph.D. Assistant Professor of Medicine, Division of Medical Genetics University of Pennsylvania School of Medicine Philadelphia, PA 19104 (215) 662-4740 cfriedri@mail.med.upenn.edu This message was originally submitted by smeltzer@UMABNET.AB.UMD.EDU to the HUM-MOLGEN list at NIC.SURFNET.NL. We are looking for RNAs and high-molecular-weight DNAs from early endometrial premalignant lesions as well as frank endometrial tumors. They should be of relatively high (50% or greater) neoplastic cell purity. Please email smeltzer@umabnet.ab.umd.edu. Thank you. Stephen J. Meltzer, M.D. Professor of Medicine, Pathology, Molecular and Cell Biology Program, and Greenebaum Cancer Center University of Maryland School of Medicine 22 S. Greene St., Room N3W62 Baltimore, MD 21201 phone: (001)-410-706-3375 fax: (001)-410-328-6559 email: smeltzer@umabnet.ab.umd.edu **************************** Reply-To: Barbara <Tomassini@utovrm.it> comments: ------------------------------------------------------------ Defects in Facc are the cause of one of the four complementation groups of Fanconi Anemia (FA). Does anybody know if an antibody against human Facc protein has been isolated and characterized? ------------------------------------------------------------ ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************