HUM-MOLGEN archive: BIOT: September 1997

New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 4250 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! -Please send high quality messages only, including full name, address and purpose. -Please use the appropriate TOPIC subject heading in your message. -Please state non-trivial questions only. -Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Arthur Bergen (BIOT editors) This BIOT message contains: 1) Help in finding a benchtop PCR hood with UV light. 2) Can two female ovums be combined for the purpose of creating an embryo? 3) The use of antisense oligonucleotides in sheep. 4) A request for information on "recanconstat" 5) A new WEB site for Hearing, Seeing, and Smelling the World. 6) Information request for any special genetic qualities of native Americans 7) A need for information on XIST. 8) A researcher would like to find some PT430 9) Is there a relationship between the process of protein synthesis and the CMT genetic disease 10) A request for information on the names an addresses of Institutions performing sequencing of the reverse transcriptase. 11) A request for information on teratogenic effects of alcohol. 12) Help in defining what role mitochondrial-DNA less cells have on hydrogen peroxide levels. 13) How does one isolate genomic DNA from paraffin-embedded tissue sections. 14) A new WEB site for PCR and multiplex PCR guidelines. ***************************************************************************** Hello: I've been trying to find a benchtop PCR hood with UV light. Could anyone give me any suggestions? (brand names, comments?) Thanks Dr. Juan Carlos Morales Juan Carlos Morales <morales@CUBSPS.BIO.COLUMBIA.EDU> ***************************************************************************** What would the process entail if there were to be the joining of two female ovums for the purpose of creating an embryo. What equipment is used to split and join the cells to start meiosis? Is there anyone who has or is performing this procedure? Greg Douglas Gregdoug@aol.com ***************************************************************************** Dear colleagues: I'm thinking of using an antisense oligonucleotide to study the effect of the inactivation of an autocrine factor in sheep embryos. In doing so, I should be able to inactivate the function of this factor at the mRNA level and measure the effects of its non-expression. Do you think this will work? What other methods should I use to evaluate the lack of an autocrine factor upon cells? Thanks for your suggestions. A. Crespo PhD Student University of Kansas Med. Center acrespo@kumc.edu **************************************************************************** A friend of mine has heard about a new experimental drug in Germany called "recanconstat" Any information you can give me on this would be appreciated. Thank you ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: ttyE10.ott.igs.net Remote IP address: 207.210.11.18 karenlg@igs.net (Karen Gilroy) ***************************************************************************** >From the Howard Hughes Medical Institutions: Hearing, Seeing, and Smelling the World http://www.hhmi.org/senses/ A colorful, animated website, with articles and illustrations, entitled "Seeing, Hearing and Smelling the World: New Findings Help Scientists Make Sense of Our Senses." Contents include: Stunning visual illusions. How we see colors. Discovering a "deafness" gene. A view of brain scans that spy on the senses. The mystery of smell. New evidence of specialized cells in the nose that receive sexual information. Glossary. It is an educational resource valuable to biology and science teachers, as well as being interesting and informative for the general public. Thank you, Philip Rink Jr On behalf of HHMI prink@MAIL.BCPL.LIB.MD.US ***************************************************************************** Please direct me to documentation regarding the special genetic qualities of native Americans. I am interested in researching this topic. We are developing a Cultural Center and Library in Texas for intertribal studies and this information would be interesting. Thank you M. L. Harris, Ed.D. mlhinc@swbell.net ***************************************************************************** Hello, I am an Honors Genetics student looking for current insights into dosage compensation links between species (mammals, Drosophila) -Nature, March 1997. I need information on XIST and its ability to recognize the X chromosome specifically. Can anyone help? Please answer to lcruise@mustang.uwo.ca ***************************************************************************** Dear Colleagues: One of my colleagues is carrying out a study relating to the mechanisms of methotrexate(MTX) resistance in patient samples. He needs some PT430 (N-(4-Amino-4-deoxy-N10-methlpteroyl) - N-(4'-fluoresceinthiocarbamyl) - L-lysine), a fluorescent MTX analog. Can you tell me where my colleague can purchase it or if you can kindly offer some to him. If you have any questions please e-mail me at kqian@npc.haplink.co.cn or write directly to: Ye Hui, M.D. Dept. of Pediatric Hematology Xin Hua Hospital, shanghai Second Meidcal University 1665 Kong Jiang Road Shanghai, 200092 People's Republic of China ***************************************************************************** What is the relationship between the overall process of protein synthesis and the CMT genetic disease? Thanks Khaldoun Aboulhosn aboulhosn@HOTMAIL.COM ***************************************************************************** Dear Editors, Can you provide me with names and addresses of Institutions performing sequencing of the reverse transcriptase and the protease gene within the European Community ? I am looking for companies with reasonable prices and in a close vicinity to Germany: University Hospital Eppendorf, Martinistr. 52 D-20246 Hamburg GERMANY stellbri@uke.uni-hamburg.de ***************************************************************************** I am currently engaged in researching the latest findings regarding the teratogenic effects of alcohol in relation to genetics and subsequent behavioral effects. Any information/referencing concerning this topic would be appreciated. Psychology Department @ Lakehead University, Care of Dr. Paul K. Satinder 955 Oliver Road S.W@Rocketmail.com ***************************************************************************** Fellow colleagues, I am presently working on a paper that focuses on the role mitochondrial-DNA less cells have on hydrogen peroxide levels in avian cell lines. Since it is logical to assume that H2O2 production would be an abberrant result of an error at the terminal electron acceptor of the electron transport system, can you predict any other potential sources? In a past study, individuals who received aspirin (a known ETS-uncoupler) showed a lower incidence of colon cancer. How could one relate aspirin's uncoupling effect to a lower incidence of colon cancer? Thank you for your support. Yours truly, Andrew Pilon Send Ideas To:pilona@muss.cis.mcmaster.ca ***************************************************************************** I would like to receive a good protocol to isolate genomic DNA from paraffin-embedded tissue sections. Dept Immunology Marques de Valdecilla University Hospital 39008 Santander Spain hospif01@sarenet.es ***************************************************************************** Dear Sirs, A new web site has recently been developed at Indiana University. This site is dedicated to helping researchers and technologists solve various problems they might encounter while performing PCR and multiplex PCR reactions. This link includes many pages, each dedicated to one aspect or parameter of the PCR reaction. There are about 45 gel pictures, all described and discussed, to help for a better understanding of the various parameters. The URL is: "http://medgen.iupui.edu/~ohenega/PCR.html" For more information, please contact: Octavian Henegariu, M.D. E-mail: ohenega@iupui.edu URL: php.iupui.edu/~ohenega ______________________________ Octavian Henegariu, M.D. Indiana University, IB #130 975 W Walnut Street Indianapolis, IN 46202-5251 Tel: 317-274-1054; Fax:317-278-1616 E-mail: "ohenega@iupui.edu" URL: "medgen.iupui.edu/~ohenega " ***************************************************************************** ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************