HUM-MOLGEN archive: BIOT: various dec 1996

New BIOTechnology and molecular biology related messages. Please send high quality messages only related to the correct TOPIC. Other messages may be refused or get lost without further notification. Please respons by private E-mail only. Happy new year! Arthur Bergen ************************************************************************ Subject: (Fwd) HLA-Typing Thomas.Dick@Ac.cybercity.de (Thomas Dick) sent the following comments: Hello, we want to investigate the genomic sequence of the HLA-DRB1- gene of some of our patients to elucidate the HLA association of autoantibodies occuring in these patients. For this purpose we need the genomic intron-sequence of the HLA-DRB1-gene especially in front of the secound exon to construct a primer to start using patients DNA as template. Can anybody tell us were we can find the sequence data ? Thank you in advance Dr. Thomas Dick, Rheumafroschungsinstitut Aachen, Germany E-Mail.: Thomas.DICK@AC.CyberCity.de Voice.: Germany +49 241 /6096-4061 or. 4060 Fax.: Germany +49 241 /6096-1964 Paper-Mail.: Dr. Thomas Dick, Rheumaforschungsinstitut Haupstr.21, D-52066 Aachen, Germany Server protocol: HTTP/1.0 Remote host: susi.Informatik.RWTH-Aachen.DE Remote IP address: 137.226.144.51 ************************************************************************ Subject: (Fwd) Protein sequencing From: =?iso-8859-1?Q?Ren=E9?= Zimmermann <rzimmer@KERCKHOFF.MPG.DE> Subject: Protein sequencing This message was originally submitted by rzimmer@KERCKHOFF.MPG.DE to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear colleagues, does anybody know a company doing commercial protein sequencing ? Any information is appreciated. Thanks R. Zimmermann Dr. Rene Zimmermann Molecular Cardiology Laboratory Max-Planck-Institute Dept. of Experimental Cardiology Benekestrasse 2 D-61231 Bad Nauheim GERMANY Tel.: (0)6032/705-404 Fax: (0)6032/705-419 e-mail: rzimmer@kerckhoff.mpg.de ************************************************************************ From: Alex Dobrovic <adobrovic@MEDICINE.ADELAIDE.EDU.AU> This message was originally submitted by adobrovic@MEDICINE.ADELAIDE.EDU.AU to the HUM-MOLGEN list at NIC.SURFNET.NL. We are looking for a protocol using TdT and P-33 dATP to label antisense oligonucleotides for in situ hybridisation of tissue sections.Any comments onthe length of tail that's desirable, the need to use cold nucleotides etc would be appreciated. Alexander Dobrovic, Ph. D. Chief Scientist Department of Haematology-Oncology The Queen Elizabeth Hospital Woodville, SA 5011, Australia Affiliate Senior Lecturer Department of Medicine University of Adelaide Tel 61-8-8222 6884 Fax 61-8-8222 6046 (note new numbers as of August 1996) ************************************************************************** From: Amin El-Nashar <ask@INTOUCH.COM> Reply to: Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL> This message was originally submitted by ask@INTOUCH.COM to the HUM-MOLGEN list at NIC.SURFNET.NL. plase informate us about if you have a researsh about relation between oncogenes (myc family ) and brain tumours fax :3612221 email : ask@intouch.com **************************************************************************** >From dfarkas@beaumont.eduMon Jan 13 10:13:18 1997 From: "Daniel H. Farkas, PhD" <dfarkas@beaumont.edu> To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@NIC.SURFNET.NL> Subject: Trisomy 18, trisomy 13 This message was originally submitted by dfarkas@BEAUMONT.EDU to the HUM-MOLGEN list at NIC.SURFNET.NL. Last week, a child was born and lived for about an hour. There were several, severe anomalies but no diagnosis was made. Before the autopsy, liver and spleen specimens were sent to my lab for DNA Extraction and Banking (at the request and with permission of the parents). The liver DNA was badly degraded but the spleen DNA is excellent and we have over 600 micrograms of it. Cytogenetics was also requested but no cells grew. The attending suspects Trisomy 18 or 13 and would like to have a diagnosis in this case. Now that cytogenetics is not possible, does anyone out there know of a lab that can do trisomy analysis on a DNA specimen? Seems like it would take some pretty finely tuned quantitation with chromosome specific probes, perhaps by Southern blotting with an internal control. Anyone's help would be greatly appreciated. Thanks. Happy Holidays, Dan Farkas, PhD Co-director, Molecular Probe Lab Wm. Beaumont Hosp. Royal Oak, MICHIGAN, USA (810) 551-5077-phone (810) 551-3694 (fax) dfarkas@beaumont.edu ************************************************************************* From: Joe Lorenz <jlorenz@geneticid.com> Subject: CALL: nonisotopic sequencing This message was originally submitted by jlorenz@GENETICID.COM to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear colleagues: I am presently in a lab that does not utilize radioisotopes. I have been trying to sequence mtDNA PCR product using cycle sequencing and silver staining with very limited success. I was wondering whether there is anyone out there who could either help me refine my technique or advise me about other non-isotopic squencing systems. Thanks in advance. Joseph G. Lorenz, Ph.D. Analytical Genetic Testing Center 7808 Cherry Creek S. Dr., #201 Denver, CO 80231 (303) 750-2023 (303) 750-2171 fax jlorenz@geneticid.com