HUM-MOLGEN archive: BIOT, various, Feb 1996

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Arthur Bergen ************************************************************************ Date: Mon, 05 Feb 1996 21:39:06 -0500 From: Barry Mellovitz <yisber9@EARTHLINK.NET> Reply to: Human Molecular Genetics Editors <ED-MOLGEN@nic.SURFnet.nl> This message was originally submitted by yisber9@EARTHLINK.NET to the HUM-MOLGEN list at NIC.SURFNET.NL. Hi... I'm looking for protocols on mouse for looking at meg. growth or platelet increases, etc. It seems that it's not so simple to find just one assay in-vitro that will look for something that is beneficial for thrombocytopenia or even just an increase in megs. I've had the same cmpd give me many cfu-meg alone, while with IL-3 it only increased the amount of single megs in mixed colonies. I've never tried looking at dosed animals, since that precludes a large volume of cmpds to screen in a short time. But I also need to find these protocols, which I will anyway, but would like to get any help from anyone experienced in theese procedures. I've used acetylcholamine for small progenitor megs,(and know that some use it for LTC assays for amount of megs) but haven't looked at platelet numbers or size, as well as meg size. I have access to a facs, and determining numbers wouldn't be a problem, but would like to discuss more practical issues, such as which cytokines to use. I've tried a few and had very good success, but like I said, didn't look at the volume of the nucleus for the 'n' number or the amount of platelets. I'm unfamiliar with aggregation techniques or their purpose, and the issue of AFP which I believe is an autoimmune destruction of your own platelets, is complicated since the person has an excess of megs, but a great lacking of platelets. Thanks for any input, I'm also on compuserve - 74511.2067@compuserve.com Barry Mellovitz Abbott Laboratories Dept of Growth Factors ************************************************************************* >From roizes@INFOBIOGEN.FR From: Gerard Roizes <roizes@INFOBIOGEN.FR> Subject: INTEGRATED GENETICS This message was originally submitted by roizes@INFOBIOGEN.FR to the HUM-MOLGEN list at NIC.SURFNET.NL. February 6th 1996 I have received the information concerning the new genetic diagnostic technology developped by Integrated Genetics, called MASDA. I wish to get into contact with Integrated Genetics, but have no address available, nor Telephone number or email. I would be gratefull to you if you could provide me with this information. Thank you very much in advance. yours sincerely, Gerard Roizes Gerard ROIZES S.F.E.G.E UPR9008 CNRS et U249 INSERM Institut de Biologie 4 Bvd Henri IV 34060 Montpellier FRANCE Phone: (33) 67 60 11 23 Fax: (33) 67 60 94 78 email: lovelace.infobiogen.fr *********************************************************************** >From agoffinet@QUICK.CC.FUNDP.AC.BE From: andre goffinet <agoffinet@QUICK.CC.FUNDP.AC.BE> Subject: in situ PCR This message was originally submitted by agoffinet@QUICK.CC.FUNDP.AC.BE to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear Editor: I wonder whether anyone connected might have experience with using in situ PCR to reveal rare messages on frozen sections. We have currently difficulties setting up this technique and would like to spend some time abroad (in Europe) to learn the necessary skills. Andre GOFFINET Dept Physiologie Humaine FUNDP Med. School 61, rue de Bruxelles B5000 Namur, Belgium Tel: 32 - 81 - 724277 Fax: 32 - 81 - 724280 *********************************************************************** >From hsibul@EBC.EEFri Mar 8 10:31:23 1996 Date: Wed, 14 Feb 1996 16:19:57 +0200 From: Hiljar Sibul <hsibul@EBC.EE> This message was originally submitted by hsibul@EBC.EE to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear Editor, [ I hope this is the right place to get the message to the net community] I am interested in the cell line that would lack functional low-density lipoprotein receptors ( LDLR ). This would probably be derived from a patient with homozygous form of familial hypercholesterolemia ( FH ). If anyone has such a cell line or information , where it could be obtained from, please reply directly by email. I would also appreciate the complete sequence (or restriction map) of a plasmid designated pLDLR 12 or 22. Unfortunately it was not to be found in currently available vector databases. Hiljar Sibul Estonian Biocentre hsibul@ebc.ee -- Hiljar Sibul Estonian Biocentre e-mail: hsibul@ebc.ee University of Tartu phone: 372-7-420210 23 Riia St. 2400 Tartu Estonia, Europe fax: 372-7-420286 ***************************************************************************** Date: Wed, 14 Feb 1996 13:18:49 -0500 (EST) From: Eva Czarnecka-Verner <evaczar@MICRO.IFAS.UFL.EDU> Reply to: anair@gnv.ifas.ufl.edu Hello to everybody: I am very interested in obtaining plasmids containing coding sequences for the full length LexA DNA binding domain, as well as, LexA DNA binding site present in multiple copies. Please, let me know if there is someone out there who can help. Thank you. Eva Verner Microbiology Department University of Florida Bldg. 981, P.O. Box 110700 Gainesville, Fl 32611-0700 ************************************************************************* REPLY >From scott_jokerst@DATA-TRANSPORT.COM Subject: Re: Restriction map analysis This message was originally submitted by scott_jokerst@DATA-TRANSPORT.COM to the HUM-MOLGEN list at NIC.SURFNET.NL. Ana, I went to Biological Data Transport's web site at: http://www.data-transport.com and queried for restriction mapping. (You can query for many life science related topics, and the site is becoming more comprehensive daily). Two companies come up out the system with that query, the first one being DNASTAR, Inc., which is offering the LASERGENE software for evaluation. A click on that link will bring you directly to DNASTAR's LASERGENE product information. Hope that helps, Scott ************** At 4:23 AM 1/29/96, Ana Margarida Ianhes wrote: >This message was originally submitted by aianhes@IMAGEM.IBILI.UC.PT to the >HUM-MOLGEN list at NIC.SURFNET.NL. > >I'm looking for a program that allows Restriction map analysis. I wish to >know where I can find it. > Thanks for your attention > > Ana Margarida Ianhes >************************************************************************* ---> R. Scott Jokerst scott_jokerst@data-transport.com ---> ---> Biological Data Transport http://www.data-transport.com ---> ---> 510-648-8229 510-648-8279 (FAX) --->