HUM-MOLGEN archive: BIOT: various; Jan 1996

Note from the editor: New BIOT messages from Jan 1996! The BIOT TOPIC is open for requests and offering of protocols, material, vectors, clones and biotechnical and molecular inquiries as well as sharing of expertise in the molecular genetic and -biological field. Also, information about new techniques, methods as well as grants or awards for research projects may be placed here. Please respond by private E-mail only, unless your response is of general interest to the HUM-MOLGEN community. Arthur Bergen ************************************************************************** From: IN%"twalker@csu.edu.au" Subj: RE: c-myc proteins levels by flow cytometry This message was originally submitted by 9000114@WIS.RIV.CSU.EDU.AU to the HUM-MOLGEN list at NIC.SURFNET.NL. Hello. I require your assistance. I wish to quantitate the protein levels of the oncogene c-myc in tumour tissue from an in vivo rat model using flow cytometry. Has anyone had experience or can direct me to a site where I might ascertian this information. Regards Todd L. Walker Charles Sturt University, Australia. twalker@csu.edu.au *************************************************************************** From HSwiger@AOL.COMWed Feb 14 10:13:40 1996 Date: Thu, 28 Dec 1995 19:40:28 -0500 From: HSwiger@AOL.COM Subject: Intracytoplasmic Sperm Injection This message was originally submitted by HSwiger@AOL.COM to the HUM-MOLGEN list at NIC.SURFNET.NL. Hi, For a while I have been attempting to obtain detailed information/current research on ICSI. If you have more information on this subject please contact my e-mail or phone me at 619-747-4781 or fax at 619-747-0625. Please send this information expedititiously. If you prefer to mail this info: 3913 Summer Way Escondido, CA 92025 I really appreciate your time and effort in these matters. Holly S. ************************************************************************* From becker@MAILER.UNI-MARBURG.DEWed Feb 14 10:14:48 1996 Date: Wed, 10 Jan 1996 19:26:10 +0100 From: "Becker Stephan Dr." <becker@MAILER.UNI-MARBURG.DE> This message was originally submitted by becker@MAILER.UNI-MARBURG.DE to the HUM-MOLGEN list at NIC.SURFNET.NL. We are looking for information about the vector pJL3 encoding the bacteriocin-release-protein. Can somebody tell me the name and address of the supplier? Please send your message to Stephan Becker "Becker@Mailer.uni-marburg.de" Many thanks in advance. Stephan **************************************************************************** From NSMART@RVC.AC.UKWed Feb 14 10:16:45 1996 Date: Wed, 17 Jan 1996 11:07:01 +0000 (GMT) From: Nicola Smart <NSMART@RVC.AC.UK> This message was originally submitted by NSMART@RVC.AC.UK to the HUM-MOLGEN list at NIC.SURFNET.NL. Hello, I have recently started work on down-regulation of PKC in signal transduction and am intending to introduce random mutations into a PKC sequence using error-prone PCR. Can anyone advise me on how to carry out such work, since after extensive searching I have yet to find anybody using this technique - naturally, all literature describes working towards error-free PCR! Any advice will be greatly appreciated. Nicola nsmart@rvc.ac.uk Royal Veterinary College Royal College Street London NW1 0TU *************************************************************************** From selim@CBU.BAYAR.EDU.TRWed Feb 14 10:18:26 1996 Date: Fri, 19 Jan 1996 15:43:19 +0300 From: Selim Uzunoglu <selim@CBU.BAYAR.EDU.TR> Subject: call: the problem of chromosome number in meiosis This message was originally submitted by selim@CBU.BAYAR.EDU.TR to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear colleaques, One colleaque, cytogeneticist, said that, primary spermatoid starts to divide with 46 chromosomes and end up with 23 chromosomes(haploid) in meiotic division according to his readings and observations. But from the perspective of molecular genetics, I believe that it starts to divide after doubling genetic material(ie;92 chromosome, it becomes first tetraploid). In the end of meiosis, four haploid sperm or one oocyte form. He says that he could not get any experimental photo showing 92 chromosome(tetraploid) containing cell stage in chromosome studies. I told him, molecular geneticist and cytogeneticist are studying different levels of genetic material and therefore their concepts and language are not the same often. These are my questions: 1- How can we match this two level information in coherent way? How much valid this cytogenetic information in clinical cases without contradiction with molecular genetic data? 2- Are there any information or publication containing experimental photos related to chromosomal number of each stage of meiotic division in human spermatogenesis or oogenesis? 3- As a molecular geneticist, is it still reliable to use concepts such as chromosome, chromatid,chromatin ,which their meaning have historical dimension. Thank you very much for all contribution made in advance regards selim email: selim@cbu.bayar.edu.tr ************************************************************************ From aianhes@IMAGEM.IBILI.UC.PTWed Feb 14 10:19:37 1996 Date: Mon, 29 Jan 1996 13:23:58 +0100 From: Ana Margarida Ianhes <aianhes@IMAGEM.IBILI.UC.PT> Subject: Restriction map analysis This message was originally submitted by aianhes@IMAGEM.IBILI.UC.PT to the HUM-MOLGEN list at NIC.SURFNET.NL. I'm looking for a program that allows Restriction map analysis. I wish to know where I can find it. Thanks for your attention Ana Margarida Ianhes ************************************************************************* From Gary.Franklin@DEVBIOL.UU.SEWed Feb 14 10:20:23 1996 Date: Mon, 29 Jan 1996 18:36:41 +0100 This message was originally submitted by Gary.Franklin@DEVBIOL.UU.SE to the HUM-MOLGEN list at NIC.SURFNET.NL. To all fellow HUM-MOLGEN subscribers: Hello ! I am currently in need of two things for a coming project and would be grateful if anyone "out there" could provide me either with the materials themeselves, or suggest a possible source which I could contact. The first thing is a CAT reporter plasmid containing the HSV TK promoter (preferably with lots of nice cloning sites); I haven't managed to find a commercially available construct sold by any of the "usual" sources :- maybe you know of one ? The second thing I would be extremely interested in would be a plasmid for use in transfection assays containing two different reporter genes (say CAT and ßGal) and intervening cloning sites: if such a plasmid had the two reporter genes arranged in opposite orientations, so much the better. If anyone has any suggestions, I would be very happy to hear from you ! Gary Franklin ************************************************************************* From e013mr02@AREA.BA.CNR.ITWed Feb 14 10:20:51 1996 Date: Wed, 31 Jan 1996 18:13:11 +0100 From: Mariano ROCCHI <e013mr02@AREA.BA.CNR.IT> This message was originally submitted by e013mr02@AREA.BA.CNR.IT to the HUM-MOLGEN list at NIC.SURFNET.NL. I am looking for a cosmid library specific for chromosome 2. Grateful for any indication. Mariano Rocchi Dr. Mariano Rocchi Istituto di Genetica Via Amendola 165/A 70126 Bari - Italy tl +39-80-544.3371 fax +39-80-544.3386 e-mail m.rocchi@area.ba.cnr.it ****************************************************************************