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  Bergen (ioi): BIOT: various nov 1996  
   

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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL>
Subject: BIOT: various nov 1996
From: "Bergen (ioi)" <A.A.BERGEN@AMC.UVA.NL>
Date: Fri, 6 Dec 1996 17:12:12 +0100

New BIOTechnology and Molecular biology related issues. There are both
a lot of requests for help and a lot of collaboration or assistance in
various fields!

Please send high-quality messages only with the appropriate TOPIC subject
heading. Low-quality messages and message not related to the proper
field may be refused without further notification.

Please respond by private E-mail only.

Have a nice day

Arthur Bergen

********************************************************************


Reply-to:      "A.PAVAN PRAKASH" <scip5111@leonis.nus.sg>
From:          "A.PAVAN PRAKASH" <scip5111@leonis.nus.sg>
Subject:       call

scip5111@leonis.nus.sg (A.PAVAN PRAKASH) sent the following comments:

------------------------------------------------------------
SIR,
   IAM FACING PROBLEMS WITH HPLC, I WANT TO SEPARATE OUT DNA BASES
AND QUANTIFY THEM FOR THE LEVELS OF METHYLCYTOSINE.
THE COLUMN IAM USING IS LICHROSORB RP18(MERCK), AND THE SOLVENT FOR
ISOCRATIC ELUTION IS 5mM PHOSPHORIC ACID IN 5% METHANOL, THE FLOW RATE
IS 1.75ML/MIN. IAM DISSOLVING MY STANDARDS IN WATER. IAM FINDING
IT DIFFICULT TO SEPARATE, I HAVE NO IDEA WHAT IS GOING WRONG.
THE INSTRUMENT IS PERFUSION CHROMATOGRAPHY(BIOCAD).PLEASE LET ME KNOW
IF THERE IS ANYTHING WRONG IAM DOING AND CAN BE CORRECTED.
THANKING YOU.
A.PAVAN PRAKASH.

************************************************************************

From:          ammn001@MED1.BMI.AC.CN
Subject:       help
To:            Multiple recipients of list

Hope get the sequence of pseudomonas exotoxin gene or protein.

************************************************************************

Subject: (Fwd) CALL: Parthenogenetic trigger

From:          Cari Watson <a14peace@traveller.com>
Subject:       CALL: Parthenogenetic trigger


a14peace@traveller.com (Cari Watson) sent the following comments:

------------------------------------------------------------
I have been told that in the UK, experiments in human  parthenogenetic m
itosis were successful when the ovum was in the presence of boar sperm.
Can anyone verify this or any other successful human
arthenogenesis experiments.  I am not a mad scientist who would abuse this
knowledge;  beside a strong personal interest, I simply require
validating documentation for a paper I am writing with this
subject as a side topic.  Therefore it is important that any
journal entry specs be included.  I have been hunting elsewhere

Regards,
Cari Watson


------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: dyn_03_37.hsv.tis.net
Remote IP address: 206.154.246.164

************************************************************************

From:          Cindy Utley <utley@genvec.com>
Subject:       GMP


utley@genvec.com (Cindy Utley) sent the following comments:

------------------------------------------------------------
Looking for information on companies that can manufacture
naked DNA under GMP.  Any leads?
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: utley.genvec.com
Remote IP address: 207.86.52.67

************************************************************************

This message was originally submitted by indranet@INDO.NET.ID to the HUM-MOLGEN
list at NIC.SURFNET.NL.

Dear Sir,
I have a strain E COLI GENOTYPE: recA13 ara-14 proA2 LacxY1
I want to know the meaning/description of :
1. "13" in recA13
2. "-" and "14" in ara-14
3. "x" in LacxY1
Thanks,
Best regard, looking forward to hear from you all.
Yours sincerely,
dr. Indrayana NS
Email: indranet@indo.net.id
Fax: 62 31 5322472
Airlangga School of Medicine
Mayjen.Prof.Dr.Moestopo 47 Str.
Surabaya
Indonesia
************************************************************************

From:          Grant Morahan <morahan@wehi.edu.au>
Subject:       Reduced fertility by transgene integration
o:            Multiple recipients of list

morahan@wehi.edu.au (Grant Morahan) sent the following comments:

------------------------------------------------------------
Hello all,

    Is anyone interested in a line of transgenic mice I have which show reduced
fertility?

 These mice have a transgene encoding a T cell receptor (cf PNAS 88:11421,
1991).  Another line with the same transgene and on the same genetic background
does not have the same fertility problems.  Over the course of several years
working with these mice, the average litter size would be around 3-4 in the
former line, whereas the normal litter size (around 8) is found in the other
line. The litter sizes decreased further when we attempted sib mating to obtain
a homozygous line.

 I realise that the effect is not a very large one, but I can no longer keep
this line in production.  Therefore, I hope that I could find someone interested
in characterising it further or else I will have to abandon it.

 Please let me know by direct email whether you know anyone who may be
interested in these mice.

    Regards, Grant

Grant Morahan, Ph.D.
The Walter and Eliza Hall Institute of Medical Research,
Melbourne, Victoria 3050, AUSTRALIA

------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: wehie.wehi.edu.au
Remote IP address: 128.250.252.67

************************************************************************

Reply-to:      Insoo Kim <iskim@lgchem.powernet.co.uk>
From:          Insoo Kim <iskim@lgchem.powernet.co.uk>
Subject:       Diagnosis


iskim@lgchem.powernet.co.uk (Insoo Kim) sent the following comments:

------------------------------------------------------------
Dear Sir/Madam
I am a PhD student of Cranfield Biotechnology Centre in UK.
I am interested in Porphyrias especially diagnosis of it
I want to develope a diagnosis tool of porphyrias using electrochemical methods.
Before starting my project, I want to know general methods of diagnosis of
porphyrias.
Please give me information of those or sources.
Thanks a lot.


Insoo Kim
Cranfield Biotechnology Centre
Cranfield University
Cranfield
Bedford
MK43 0AL
UK
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: power5.power.net.uk
Remote IP address: 195.60.0.5
************************************************************************

Reply-to:      Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL>
From:          Jose Mejia <jmejia@PO-BOX.MCGILL.CA>

This  message  was  originally  submitted  by  jmejia@PO-BOX.MCGILL.CA  to  the
HUM-MOLGEN list at  NIC.SURFNET.NL.

Hi everyone:
I was invited to collaborate with some archeologists and they send some
human bones to me. I need to know what are the best methods in order to get
DNA out of those bones and which might be the best markers to genotype
them. The archeologists are interested in knowing if the bones belong to
the same group, family etc.
Thank you for any information or reference.
Jose Mejia
jmejia@po-box.mcgill.ca
fax: (514) 398-4370.

************************************************************************

Date:          Tue, 3 Dec 1996 09:37:13 +0100

This message was  originally submitted by Markus.Stumm@MEDIZIN.UNI-MAGDEBURG.DE
to the HUM-MOLGEN list at NIC.SURFNET.NL.

Dear colleagues

we are starting a project on prostata carcinoma and hypernephroma. For
this project it is necessary to culture cells from these tumors. If
some of you have experience on cell culture and cytogenetic techniques
of carcinoma cell lines, it would be helpful for us to know:

1. How to start cell cultures from these solid tumors (mechanical and
or enzymatical disaggregation).
2. Short-term and long-term cultures.
3. Cytogenetic analysis of solid tumors.

Thank you for any help!

With best regards Markus Stumm.

Markus.Stumm@Medizin.Uni-Magdeburg.de

Dipl.-Biol. Markus Stumm
Institute of Human Genetics
Cytogenetics Unit
University Hospital
Otto-von-Guericke University Magdeburg
Germany

************************************************************************

Reply-to:      Maciej Stopa <smaciej@amedec.amg.gda.pl>
From:          Maciej Stopa <smaciej@amedec.amg.gda.pl>

Hi,


I am looking for a few DNA from the human cell lines such as:
- MCF-7
- SK-BR-3
- HT-29
- SW 480
Above DNA are necessary as possitive controlfor my work.
If you help me, please write to me.
My e-mail: smaciej@amedec.amg.gda.pl
Mail address: Medical University of Gdansk
Dept.of Clinical Biochemistry
7 Debinki Str.
PL-80211 Gdansk, Poland
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: amedec.amg.gda.pl
Remote IP address: 153.19.64.1

************************************************************************

Subject: (Fwd) coxsackievirus

From:          Mark Donovan <Mark.Donovan@UNILEVER.COM>
Subject:       coxsackievirus


          I need to find out more information on the link between
          coxsackievirus (or related virus) and a human condition
          known as hematophagic histiocytosis, particularly showing
          reduced platelet numbers.

          replies to Mark.Donovan@unilever.com
************************************************************************

>From a.bergen@ioi.knaw.nlFri Dec  6 16:41:31 1996
Date: Fri, 06 Dec 1996 16:28:28 +0100 (MET)
From: Arthur Bergen <a.bergen@ioi.knaw.nl>
To: A.A.Bergen@AMC.UVA.NL
Subject: (Fwd) CALL

Reply-to:      Mavi Camarasa <MV.Camarasa@ua.es>
From:          Mavi Camarasa <MV.Camarasa@ua.es>

MV.Camarasa@ua.es (Mavi Camarasa) sent the following comments:

------------------------------------------------------------
I have a problem with the amplification of exon 8 of p53.
I am using the CLONTECH amplimer panel, and following its protocol
 exon 8 is not amplified most of times. I4ve tried to add MgCl2
but it4s not useful in all cases. What could be the reason?
Thanks a lot for your assistance.
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: ferragut.biomem.ua.es
Remote IP address: 193.145.235.66
************************************************************************

Date:          Mon, 25 Nov 1996 22:21:03 +0100
Reply-to:      miguel rivera <miguel.rivera@edp.ulaval.ca>
From:          miguel rivera <miguel.rivera@edp.ulaval.ca>

miguel.rivera@edp.ulaval.ca (miguel rivera) sent the following comments:

------------------------------------------------------------
I would like to examine by PCR a TaqI RFLP at the 5' flanking
region of the myoglobin gene. Is anyone aware of PCR primers
or sequence of this region.

------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: poste152-134.edp.ulaval.ca
Remote IP address: 132.203.134.152

************************************************************************

mgoral@bmclib2.bhs.org (Mike Goral) sent the following comments:

------------------------------------------------------------
I'm looking for some software for pcr primer design.  Has anyone out there had any
 good experience with some commercially available programs or some share ware
 you can recommend?
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host:
Remote IP address: 199.92.147.2

************************************************************************

From:          "Serge Shevchenko (WWW)" <pipa@NEWT.PHYS.UNSW.EDU.AU>

This  message was  originally  submitted by  pipa@NEWT.PHYS.UNSW.EDU.AU to  the
HUM-MOLGEN list at  NIC.SURFNET.NL.

The message:

Direct involvement of the calpain 3(n-calpain)gene mutations in limb-girdle
muscular dystrophy (LGMD-2A) is demonstrated(Cell(1995)81,27). Meanwhile the
only one publication on n-calpain molecule detection followed by its degradation
was released so far (JBC(1993)268,10593).A partial purification of a ~95 kD
thiol protease from skeletal muscle (biochemical properties of calpain 3 were
detected) has been developed in my recent post-doc fellowship.
Now I'm looking for an opportunity to continue the study.I would be obliged if
anybody could give me any advice or direction for my research.

Dr Serge Shevchenko
pipa@newt.phys.unsw.edu.au

************************************************************************

Reply-to:      silvia priori <silvia.priori@netitalia.it>
From:          silvia priori <silvia.priori@netitalia.it>
Subject:       long QT syndorme

silvia.priori@netitalia.it (silvia priori) sent the following comments:

------------------------------------------------------------
I am a cardiologist involved in long QT syndrome genotyping, I would like to share data and experience with other investigators involved in this disease.
I am also looking for the restriction enzyme BceFI (acggc 12/13) to confirma a de novo mutation on hERG in a sporadic case of LQTS. In Italy I haven't been able to find a company selling this enzyme
. Can anyone help?

Silvia G Priori, MD, PhD
University of Milan
Centro Fisiologia Clinica e Ipertensione
Via F Sforza 35
20122 Milan, italy

silvia.priori@netitalia.it
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: sli5.netitalia.it
Remote IP address: 194.177.97.249


******************************************************************************
From:          William Turner <Wjtmd@AOL.COM>
Subject:       Re: BIOT: various
X-To:          HUM-MOLGEN@nic.surfnet.nl

This message was  originally submitted by Wjtmd@AOL.COM to  the HUM-MOLGEN list
at NIC.SURFNET.NL.


My interests are in genetics of gender variations: asexuality, transvestism,
homsexuality and transsexuality. Can BIOT keep me abreast of the literature?
 WJtmd


   
 
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