HUM-MOLGEN archive: BIOT: various nov 1996

New BIOTechnology and Molecular biology related issues. There are both a lot of requests for help and a lot of collaboration or assistance in various fields! Please send high-quality messages only with the appropriate TOPIC subject heading. Low-quality messages and message not related to the proper field may be refused without further notification. Please respond by private E-mail only. Have a nice day Arthur Bergen ******************************************************************** Reply-to: "A.PAVAN PRAKASH" <scip5111@leonis.nus.sg> From: "A.PAVAN PRAKASH" <scip5111@leonis.nus.sg> Subject: call scip5111@leonis.nus.sg (A.PAVAN PRAKASH) sent the following comments: ------------------------------------------------------------ SIR, IAM FACING PROBLEMS WITH HPLC, I WANT TO SEPARATE OUT DNA BASES AND QUANTIFY THEM FOR THE LEVELS OF METHYLCYTOSINE. THE COLUMN IAM USING IS LICHROSORB RP18(MERCK), AND THE SOLVENT FOR ISOCRATIC ELUTION IS 5mM PHOSPHORIC ACID IN 5% METHANOL, THE FLOW RATE IS 1.75ML/MIN. IAM DISSOLVING MY STANDARDS IN WATER. IAM FINDING IT DIFFICULT TO SEPARATE, I HAVE NO IDEA WHAT IS GOING WRONG. THE INSTRUMENT IS PERFUSION CHROMATOGRAPHY(BIOCAD).PLEASE LET ME KNOW IF THERE IS ANYTHING WRONG IAM DOING AND CAN BE CORRECTED. THANKING YOU. A.PAVAN PRAKASH. ************************************************************************ From: ammn001@MED1.BMI.AC.CN Subject: help To: Multiple recipients of list Hope get the sequence of pseudomonas exotoxin gene or protein. ************************************************************************ Subject: (Fwd) CALL: Parthenogenetic trigger From: Cari Watson <a14peace@traveller.com> Subject: CALL: Parthenogenetic trigger a14peace@traveller.com (Cari Watson) sent the following comments: ------------------------------------------------------------ I have been told that in the UK, experiments in human parthenogenetic m itosis were successful when the ovum was in the presence of boar sperm. Can anyone verify this or any other successful human arthenogenesis experiments. I am not a mad scientist who would abuse this knowledge; beside a strong personal interest, I simply require validating documentation for a paper I am writing with this subject as a side topic. Therefore it is important that any journal entry specs be included. I have been hunting elsewhere Regards, Cari Watson ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: dyn_03_37.hsv.tis.net Remote IP address: 206.154.246.164 ************************************************************************ From: Cindy Utley <utley@genvec.com> Subject: GMP utley@genvec.com (Cindy Utley) sent the following comments: ------------------------------------------------------------ Looking for information on companies that can manufacture naked DNA under GMP. Any leads? ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: utley.genvec.com Remote IP address: 207.86.52.67 ************************************************************************ This message was originally submitted by indranet@INDO.NET.ID to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear Sir, I have a strain E COLI GENOTYPE: recA13 ara-14 proA2 LacxY1 I want to know the meaning/description of : 1. "13" in recA13 2. "-" and "14" in ara-14 3. "x" in LacxY1 Thanks, Best regard, looking forward to hear from you all. Yours sincerely, dr. Indrayana NS Email: indranet@indo.net.id Fax: 62 31 5322472 Airlangga School of Medicine Mayjen.Prof.Dr.Moestopo 47 Str. Surabaya Indonesia ************************************************************************ From: Grant Morahan <morahan@wehi.edu.au> Subject: Reduced fertility by transgene integration o: Multiple recipients of list morahan@wehi.edu.au (Grant Morahan) sent the following comments: ------------------------------------------------------------ Hello all, Is anyone interested in a line of transgenic mice I have which show reduced fertility? These mice have a transgene encoding a T cell receptor (cf PNAS 88:11421, 1991). Another line with the same transgene and on the same genetic background does not have the same fertility problems. Over the course of several years working with these mice, the average litter size would be around 3-4 in the former line, whereas the normal litter size (around 8) is found in the other line. The litter sizes decreased further when we attempted sib mating to obtain a homozygous line. I realise that the effect is not a very large one, but I can no longer keep this line in production. Therefore, I hope that I could find someone interested in characterising it further or else I will have to abandon it. Please let me know by direct email whether you know anyone who may be interested in these mice. Regards, Grant Grant Morahan, Ph.D. The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, AUSTRALIA ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: wehie.wehi.edu.au Remote IP address: 128.250.252.67 ************************************************************************ Reply-to: Insoo Kim <iskim@lgchem.powernet.co.uk> From: Insoo Kim <iskim@lgchem.powernet.co.uk> Subject: Diagnosis iskim@lgchem.powernet.co.uk (Insoo Kim) sent the following comments: ------------------------------------------------------------ Dear Sir/Madam I am a PhD student of Cranfield Biotechnology Centre in UK. I am interested in Porphyrias especially diagnosis of it I want to develope a diagnosis tool of porphyrias using electrochemical methods. Before starting my project, I want to know general methods of diagnosis of porphyrias. Please give me information of those or sources. Thanks a lot. Insoo Kim Cranfield Biotechnology Centre Cranfield University Cranfield Bedford MK43 0AL UK ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: power5.power.net.uk Remote IP address: 195.60.0.5 ************************************************************************ Reply-to: Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL> From: Jose Mejia <jmejia@PO-BOX.MCGILL.CA> This message was originally submitted by jmejia@PO-BOX.MCGILL.CA to the HUM-MOLGEN list at NIC.SURFNET.NL. Hi everyone: I was invited to collaborate with some archeologists and they send some human bones to me. I need to know what are the best methods in order to get DNA out of those bones and which might be the best markers to genotype them. The archeologists are interested in knowing if the bones belong to the same group, family etc. Thank you for any information or reference. Jose Mejia jmejia@po-box.mcgill.ca fax: (514) 398-4370. ************************************************************************ Date: Tue, 3 Dec 1996 09:37:13 +0100 This message was originally submitted by Markus.Stumm@MEDIZIN.UNI-MAGDEBURG.DE to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear colleagues we are starting a project on prostata carcinoma and hypernephroma. For this project it is necessary to culture cells from these tumors. If some of you have experience on cell culture and cytogenetic techniques of carcinoma cell lines, it would be helpful for us to know: 1. How to start cell cultures from these solid tumors (mechanical and or enzymatical disaggregation). 2. Short-term and long-term cultures. 3. Cytogenetic analysis of solid tumors. Thank you for any help! With best regards Markus Stumm. Markus.Stumm@Medizin.Uni-Magdeburg.de Dipl.-Biol. Markus Stumm Institute of Human Genetics Cytogenetics Unit University Hospital Otto-von-Guericke University Magdeburg Germany ************************************************************************ Reply-to: Maciej Stopa <smaciej@amedec.amg.gda.pl> From: Maciej Stopa <smaciej@amedec.amg.gda.pl> Hi, I am looking for a few DNA from the human cell lines such as: - MCF-7 - SK-BR-3 - HT-29 - SW 480 Above DNA are necessary as possitive controlfor my work. If you help me, please write to me. My e-mail: smaciej@amedec.amg.gda.pl Mail address: Medical University of Gdansk Dept.of Clinical Biochemistry 7 Debinki Str. PL-80211 Gdansk, Poland ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: amedec.amg.gda.pl Remote IP address: 153.19.64.1 ************************************************************************ Subject: (Fwd) coxsackievirus From: Mark Donovan <Mark.Donovan@UNILEVER.COM> Subject: coxsackievirus I need to find out more information on the link between coxsackievirus (or related virus) and a human condition known as hematophagic histiocytosis, particularly showing reduced platelet numbers. replies to Mark.Donovan@unilever.com ************************************************************************ >From a.bergen@ioi.knaw.nlFri Dec 6 16:41:31 1996 Date: Fri, 06 Dec 1996 16:28:28 +0100 (MET) From: Arthur Bergen <a.bergen@ioi.knaw.nl> To: A.A.Bergen@AMC.UVA.NL Subject: (Fwd) CALL Reply-to: Mavi Camarasa <MV.Camarasa@ua.es> From: Mavi Camarasa <MV.Camarasa@ua.es> MV.Camarasa@ua.es (Mavi Camarasa) sent the following comments: ------------------------------------------------------------ I have a problem with the amplification of exon 8 of p53. I am using the CLONTECH amplimer panel, and following its protocol exon 8 is not amplified most of times. I4ve tried to add MgCl2 but it4s not useful in all cases. What could be the reason? Thanks a lot for your assistance. ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: ferragut.biomem.ua.es Remote IP address: 193.145.235.66 ************************************************************************ Date: Mon, 25 Nov 1996 22:21:03 +0100 Reply-to: miguel rivera <miguel.rivera@edp.ulaval.ca> From: miguel rivera <miguel.rivera@edp.ulaval.ca> miguel.rivera@edp.ulaval.ca (miguel rivera) sent the following comments: ------------------------------------------------------------ I would like to examine by PCR a TaqI RFLP at the 5' flanking region of the myoglobin gene. Is anyone aware of PCR primers or sequence of this region. ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: poste152-134.edp.ulaval.ca Remote IP address: 132.203.134.152 ************************************************************************ mgoral@bmclib2.bhs.org (Mike Goral) sent the following comments: ------------------------------------------------------------ I'm looking for some software for pcr primer design. Has anyone out there had any good experience with some commercially available programs or some share ware you can recommend? ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: Remote IP address: 199.92.147.2 ************************************************************************ From: "Serge Shevchenko (WWW)" <pipa@NEWT.PHYS.UNSW.EDU.AU> This message was originally submitted by pipa@NEWT.PHYS.UNSW.EDU.AU to the HUM-MOLGEN list at NIC.SURFNET.NL. The message: Direct involvement of the calpain 3(n-calpain)gene mutations in limb-girdle muscular dystrophy (LGMD-2A) is demonstrated(Cell(1995)81,27). Meanwhile the only one publication on n-calpain molecule detection followed by its degradation was released so far (JBC(1993)268,10593).A partial purification of a ~95 kD thiol protease from skeletal muscle (biochemical properties of calpain 3 were detected) has been developed in my recent post-doc fellowship. Now I'm looking for an opportunity to continue the study.I would be obliged if anybody could give me any advice or direction for my research. Dr Serge Shevchenko pipa@newt.phys.unsw.edu.au ************************************************************************ Reply-to: silvia priori <silvia.priori@netitalia.it> From: silvia priori <silvia.priori@netitalia.it> Subject: long QT syndorme silvia.priori@netitalia.it (silvia priori) sent the following comments: ------------------------------------------------------------ I am a cardiologist involved in long QT syndrome genotyping, I would like to share data and experience with other investigators involved in this disease. I am also looking for the restriction enzyme BceFI (acggc 12/13) to confirma a de novo mutation on hERG in a sporadic case of LQTS. In Italy I haven't been able to find a company selling this enzyme . Can anyone help? Silvia G Priori, MD, PhD University of Milan Centro Fisiologia Clinica e Ipertensione Via F Sforza 35 20122 Milan, italy silvia.priori@netitalia.it ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: sli5.netitalia.it Remote IP address: 194.177.97.249 ****************************************************************************** From: William Turner <Wjtmd@AOL.COM> Subject: Re: BIOT: various X-To: HUM-MOLGEN@nic.surfnet.nl This message was originally submitted by Wjtmd@AOL.COM to the HUM-MOLGEN list at NIC.SURFNET.NL. My interests are in genetics of gender variations: asexuality, transvestism, homsexuality and transsexuality. Can BIOT keep me abreast of the literature? WJtmd