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Martin Kennedy: BIOT: Various items | ||||||||||||||||
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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL> Subject: BIOT: Various items From: Martin Kennedy <MKENNEDY@chmeds.ac.nz> Date: Fri, 1 Sep 1995 14:04:27 +1200 Date sent: 1-SEP-1995 13:58:48 This message contains 3 items: 1. Long oligo synthesis: 2. cDNA library construction from cell free systems: 3. Non radioactive YAC screening 8< ======================= cut here ======================= >8 1. Long oligo synthesis: >>>I URGENTLY need someone who is ROUTINELY SYNTHESIZING LONG OLIGOS (200-300 >>>mers) and is able to provide high quality gel-purified product PREFERABLY >>>WITHIN CANADA. > >Here's another useful url > >http://www.biosupplynet.com/bsn/ > >This Web site allows the user to search in the Cold Spring Harbor Lab Manual >Source Book product/supplier database. In the search engine just type in >oligonucleotide synthesis, and a comprehensive list of suppliers will appear >along with their phone numbers. Complete contact information can also >be found for more than 1400 suppliers. It's a great resource. > >Joan Boyce> > >CSHL Press >Phone: (516) 367-8325 >Fax: (516) 367-8432 >E-mail boyce@cshl.org 8< ======================= cut here ======================= >8 2. cDNA library construction from cell free systems: >Hi, > >Has any one tried to use "cell free system (in vitro)" to transcribe m-RNA from >genomic DNA and then construct a cDNA library from it ? Is it possible ? > >Ramnath Elaswarapu (Dr) 8< ======================= cut here ======================= >8 3. Non radioactive YAC screening >Hi! > >Has anyone tried using the non-radioactive systems to probe HIGH-DENSITY YAC COLONY filter (yeast)? >We have conducted some pilot experiments with Digoxigenin system of Boehringer-Mannheim and ECL >and Fluorescein Gene Images labelling and detection system of Amersham International. > >Our preliminary attempts have not been very successful as we get high background, non-specific >hybridisation, non-uniform signal strength and spots all over the membrane (due to alkaline >phosphatase?). There is also a major problem with the reproducibility of the results. We have >followed the protocols, precautions etc. as recommended by the manufacturers of these kits. > >I am now interested in knowing if anyone else has tried these kits for YEAST COLONY filter >hybridisations and has had any success? If a large number of replies are received, I will summarise and re-post to this group. > >Ramnath Elaswarapu (Dr) >e-mail: relaswar@hgmp.mrc.ac.uk 8< ======================= cut here ======================= >8 Cheers, Martin Kennedy, Arthur Bergen BIOT Topic editors
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