BIOT: Various items

[Martin Kennedy <MKENNEDY@chmeds.ac.nz>]

Date sent:  1-SEP-1995 13:58:48

This message contains 3 items:

1. Long oligo synthesis:
2. cDNA library construction from cell free systems:
3. Non radioactive YAC screening

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1. Long oligo synthesis:

>>>I URGENTLY need someone who is ROUTINELY SYNTHESIZING LONG OLIGOS (200-300
>>>mers) and is able to provide high quality gel-purified product PREFERABLY
>>>WITHIN CANADA.
>
>Here's another useful url
>
>http://www.biosupplynet.com/bsn/
>
>This Web site allows the user to search in the Cold Spring Harbor Lab Manual
>Source Book product/supplier database. In the search engine just type in
>oligonucleotide synthesis, and a comprehensive list of suppliers will appear
>along with their phone numbers. Complete contact information can also
>be found for more than 1400 suppliers. It's a great resource.
>
>Joan Boyce>
>
>CSHL Press
>Phone: (516) 367-8325
>Fax:   (516) 367-8432
>E-mail  boyce@cshl.org

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2. cDNA library construction from cell free systems:
>Hi,
>
>Has any one tried to use "cell free system (in vitro)" to transcribe m-RNA from
>genomic DNA and then construct a cDNA library from it ? Is it possible ?
>
>Ramnath Elaswarapu (Dr)


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3. Non radioactive YAC screening

>Hi!
>
>Has anyone tried using the non-radioactive systems to probe HIGH-DENSITY YAC
COLONY filter (yeast)?
>We have conducted some pilot experiments with Digoxigenin system of
Boehringer-Mannheim and ECL
>and Fluorescein Gene Images labelling and detection system of Amersham
International.
>
>Our preliminary attempts have not been very successful as we get high
background, non-specific
>hybridisation, non-uniform signal strength and spots all over the membrane
(due to alkaline
>phosphatase?). There is also a major problem with the reproducibility
of the results. We have
>followed the protocols, precautions etc. as recommended by the manufacturers
of these kits.
>
>I am now interested in knowing if anyone else has tried these kits for YEAST
COLONY filter
>hybridisations and has had  any success? If a large number of replies are
received, I will summarise and re-post to this group.
>
>Ramnath Elaswarapu (Dr)
>e-mail: relaswar@hgmp.mrc.ac.uk
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Cheers,

Martin Kennedy, Arthur Bergen
BIOT Topic editors