BIOT: Oligonucleotides, GFP

[Martin Kennedy <MKENNEDY@chmeds.ac.nz>]

This summary contains 2 messages:

1.  Oligonucleotide stability
2.  Green fluorescent protein


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1.  Oligonucleotide stability

From:         Todd Walker <9000114@WIS.RIV.CSU.EDU.AU>
Organization: Charles Sturt University
Subject:      Re: Oligonucleotide solubility

Dear Colleagues,

Just a quick inquiry regarding the solubility of phosphorothioate
oligonucleotides. Does anyone know if a concentration of
5.2mg/mL (5.2ug/uL) in sterile water is possible using a 15-mer oligo?

Regards
Todd L. Walker
ADBLT, BAppSci(Hons), CT(ASC), CMIAC,
Cytotechnologist / PhD Candidate
Charles Sturt University, Australia.
twalker@csu.edu.au

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2.  Green fluorescent protein

From:         Larry Suva <lsuva@BIH.HARVARD.EDU>
Organization: Beth Israel Hospital Boston MA
Subject:      Green fluorescent protein

Does anyone have information/experience (good or bad) with GFP?  We are
especially interested in activation times and stability.

Larry Suva
Beth ISrael Hospital
Boston
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Editors note:  There is a bionet newsgroup dedicated to
fluorescent proteins in molecular biology:

 bionet.molbio.proteins.fluorescent

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Martin Kennedy          Arthur Bergen
BIOTech/Molbiol editors