HUM-MOLGEN archive: CALL: various

This CALL contains 6 submessages: 1) CALL: FIXED HUMAN EMBRYO AND ZYGOTE BANK 2) CALL: Sexing Beagle fetuses 3) CALL: BEAN 4) CALL: Teaching genetics subjects 5) CALL, COMP: Phylogenetic trees 6) REPLY: Autosomal dominant exudative retinopathy Good CALLs! Arthur Bergen (owner HUM-MOLGEN) ****************************************************************************** This message was originally submitted by jbamfort@GPU.SRV.UALBERTA.CA to the HUM-MOLGEN list at NIC.SURFNET.NL. We are proposing to construct a human embryo and zygote bank of fixed (NOT live) tissue. The purpose of this bank would be to make available material for in situ hybridization studies of gene expression in early human conceptuses. The material would be available as sections that express mRNA. The gestational age of each embryo or zygote would be known. Interval sections are stained with H and E so that a good knowledge of the anatomy of the material would be available. This should be useful for individuals studying the temporal and regional expression of developmentally expressed genes and gene products. We acknowledge that there are major ethical concerns with this type of proposal. We are discussing this with bioethicists and several Research and Ethics Boards. (For Dr.Bieseker's information, these are the Canadian equivalent of the American IRBs and have similar powers and mandate). Clearly, without such review a proposal such as this can not proceed. We would like to know how many people in the Research Community would use such material if it were ethically approved and available within, say, 2 years. Steve Bamforth. jbamfort@gpu.srv.ualberta.ca ************************************************************************* From: IN%"bui@gen.ks.se" "The-Hung Bui, M.D." 4-JUL-1995 13:27:00.46 Subj: CALL: sexing Beagle fetuses bui@gen.ks.se (The-Hung Bui, M.D.) sent the following comments: ------------------------------------------------------------ I would appreciate suggestions/help to get primer sequences specific for the Y chromosome in Beagle dog. This is for an experimental series addressing the feasibility of in utero hematopoietic stem cell transplantation. The project has been approved by our Ethics Committee. ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: gate1.ks.se Remote IP address: 193.12.146.100 *************************************************************************** From: IN%"march004@mc.duke.edu" "Douglas Marchuk" 10-JUL-1995 20:32:04.35 Subj: Bean (Blue rubber bleb nevus syndrome march004@mc.duke.edu (Douglas Marchuk) sent the following comments: ------------------------------------------------------------ We have genetic linkage for a family that we feel has blue rubber bleb nevus (Bean) syndrome and are searching for other families to confirm this result. We are hoping to locate clinical associates who would be willing to ascertain and draw bloods from their families. We would check the linkage here in my lab. This would be on a collaborative basis, with joint authorship on any paper resulting from this work. Our large family shows a lod score of over 9.0 with the most closely linked marker. Thus we are confident of the linkage but would like to look at more families with a similar clinical phenotype. Please call Doug Marchuk, Dept of Genetics Duke University, Durham, NC 27710 phone 919 684-3290 fax 919 684-2790 ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: marchuk01.mc.duke.edu Remote IP address: 152.3.74.11 **************************************************************************** > Date: Sun, 9 Jul 1995 11:04:51 +0200 > From: lvreiner@weizmann.weizmann.ac.il > Subject: posting a message? > To: zollmann.1@osu.edu > Message-Id: <CEC76807949F3955A5@phem6.acs.ohio-state.edu> > Mime-Version: 1.0 > Content-Type: text/plain; charset="us-ascii" > X-Envelope-To: mkh536@cks1.rz.uni-rostock.de > > The instructions for posting a message was not clear to me, therefore I am > forwarding you my message. > > Next year I shall be teaching a course for graduate students focused on > human molecular genetics, including the human genome project. I am > planning exercises that students will have to use different databases, such > as, GDB, online McKusick, sigma etc. in order to solve them. I believe > that the databases are one of the main tools in our research today. I'd > appreciate suggestions for specific questions. > > Sincerely, > > Orly Reiner, Ph.D. > Department of Molecular Genetics and Virology > Weizmann Institute of Science > Rehovot 76100 > Israel > > Phone: (972) 8 342319 > FAX: (972) 8 344108 > e-mail: lvreiner@weizmann.weizmann.ac.il > > > ************************************************************************* From: IN%"ED-MOLGEN@nic.SURFnet.nl" "Human Molecular Genetics Editors" 11-JUL-1995 14:37:27.47 To: IN%"ED-MOLGEN@nic.SURFnet.nl" "Multiple recipients of list ED-MOLGEN" CC: Subj: CALL,COMP: phylogenetic tree Date: Tue, 11 Jul 1995 13:43:45 GMT+0100 From: I Meulenbelt <I.MEULENBELT@PG.TNO.NL> Subject: CALL,COMP: phylogenetic tree To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@nic.SURFnet.nl> This message was originally submitted by I.MEULENBELT@PG.TNO.NL to the HUM-MOLGEN list at NIC.SURFNET.NL. I have been analyzing polymorphic DNA haplotypes with 7 RFLP markers. I would like to hypothesize on the evolutionary relations and construct a phylogenetic tree. I would appreciate it very much if someone could provide me with computer programs to perform such phylogenetic analysis or if someone could provide me with addresses of people working in this field. Thank you very much in advance Ingrid Meulenbelt ************************************************************************** Subj: REPLY: CALL: various This message was originally submitted by cfriedri@WELCHLINK.WELCH.JHU.EDU to the HUM-MOLGEN list at NIC.SURFNET.NL. The gene for autosomal dominant exudative vitreoretinopathy (AKA Criswick-Schepens syndrome) has been mapped, but a linkage study in one family we have studied shows no linkage to the previously assigned location, and weak evidence suggesting linkage on a different chromosome. This suggests genetic heterogeneity. We are looking for other families to test this. Chris Friedrich, M.D., Ph.D. Voice (410) 614-2521 Lipid Research-Atherosclerosis Unit Fax (410) 955-1276 Dept. of Pediatrics, CMSC 6-104 Voice mail (410) 614-1030 Johns Hopkins University School of Medicine 600 North Wolfe Street Baltimore, MD 21287 cfriedri@welchlink.welch.jhu.edu