BIOT: Oligonucleotide questions

[Martin Kennedy <MKENNEDY@chmeds.ac.nz>]

Two BIOT messages:

(1) Very Long Oligos
(2) Oligo Affinity Constants

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(1) VERY LONG OLIGOS!!!!!!

I URGENTLY need someone who is ROUTINELY SYNTHESIZING LONG OLIGOS (200-300
mers) and is able to provide high quality gel-purified product PREFERABLY
WITHIN CANADA.

Jamal Nasir, Ph.D.
Dept. of Medical Genetics
N.C.E. Building
421-2125 East Mall
University of British Columbia
Vancouver, B.C. V6T 1Z4
CANADA

TEL. 604-822 1241
FAX. 604-822 9238

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(2) Affinity constant of oligonucleotides

Hello all,

Does anyone have any information on the affinity constant of oligonucleotides?

I am working on some eighteen bases long oligonucleotides and it would be a
great help if somebody can give me some ballpark figures or direct me to
some databases. I have heard of figures like ten to the power of twentieth
mole per litre. Is it anywhere near the truth?

Thanks.

Fred



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Frederick W Chu,                        Email: f.chu@ucl.ac.uk
Division of Molecular Endocrinology,      Tel: +44 (0)171-636-8333 ext 3225
UCL Medical School, Mortimer Street,      Fax: +44 (0)171-580-2737
London W1N 8AA, UK.

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