HUM-MOLGEN archive: CALL, BIOT: various

This BIOT CALL contains 3 submessages: 1) CALL for DNA banking experience 2) CALL for "promotor finder" experience 3) CALL : RNA binding proteins The CALL section is open for CALLs for collaboration, OFFERs of assistance, CALLs for information or other help. The BIOT section primarily discusses technical problems, companies and funding and other subjects related to BIOTechnology/Molecular Biology. Please REPLY to these CALLs by person to person E-mail, and not to HUM-MOLGEN, unless your answer is of general interest to the entire HUM-MOLGEN digital web. Good CALLs ! Arthur Bergen ************************************************************************ Subj: CALL- DNA banking This message was originally submitted by jeff_kant@PATH1A.MED.UPENN.EDU to the HUM-MOLGEN list at NIC.SURFNET.NL. Subject: Time:7:21 PM OFFICE MEMO CALL: DNA banking Date:8/1/95 For those with banking experience. A colleague would like to establish a bank for future DNA extraction from bloods at minimum effort. He favors either a commercial kit or sucrose-triton lysis. What is people's experience with long term storage of partially prepared DNA, or other recommended suggestions for such a project. Jeffrey Kant University of Pennsylvania jeff_kant.labmed@path1a.med.upenn.edu ***************************************************************************** Subj: call : clontech promotor finder This message was originally submitted by WMGPJEA@CARDIFF.AC.UK to the HUM-MOLGEN list at NIC.SURFNET.NL. I am using the Clontech kit, Human Promotor Finder which is a vectorette PCR method for amplifying into regions of unknown sequence. I have had problems with the protocol and have only been able to get 1 of the 5 positive controls included in the kit to work. Has anyone else used this method? Did you get the controls to amplify? Dr Philip Asherson Institute of Medical Genetics Heath Park Cardiff CF4 4XN e-mail: asherson@cardiff.ac.uk ********************************************************************* Subj: RNA binding proteins This message was originally submitted by VOGT@NOVSRV1.PIO1.UNI-HEIDELBERG.DE to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear experts I'm urgently looking for conserved sequence boxes of genes coding for RNA binding proteins. Has anybody suggestions how to screen own sequence data effiently with all known conserved RNA binding motifs in the data base. Thanks a lot for any hint!!!!!!!!!!!!!!! Peter ********************************************* Dr. rer. nat. habil. Peter H. Vogt Section Molecular Human Genetics Institute Human Genetics & Anthropology Unuversity Heidelberg Im Neuenheimer Feld 328 D-69120 Heidelberg Germany FAX: (6221) 565332 Phone: (6221) 563924 main interests: @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ genetic aspects of spermatogenesis human male infertility and ICSI Y genes involved in spermatogenesis (AZF loci) @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @