A) Aquatic Plant Agronomy & Environmental Remediation Using Closed-Loop Animal-Fed Fertilized Pond Bed Culturement. Water hyacinth, duckweed and seagrass. These plants are to be assessed as feeds with livestock as to: a) acceptability/palatability, b) nutritive value (NV) specifically N balance and c) postharvest processing as hay or silage and solid-substrate fermentation (SSF) biomass, with the potential for high-quality (HQ) feedstock as feed and for biofuel manufacturing breakthroughs in terms of economics. All classes of livestock including pigs, chickens, goats, sheep, cattle and dairy may be fed on these aquatic-based forages.

 

B) Feeding Hi-Sugar Forages Bred Using Marker Assisted Selection (MAS) and Other non-GMO Approaches Including Protoplast Fusion & Protein Protection by Heat Damage and Use of Tannins for Browse Trees and Shrubs. These are all to be further verified with the aid of molecular probes consisting of protein nucleic (PNA)-B12 carriered gene silencing techniques as to the mechanisms at the molecular level on rumen microbial mitogenesis as further theorized by D. A. Flores (principal investigator) based on water-soluble carbohydrates (WSC) and pre-formed amino acids (PFAA) effects on cell-cycle regulatory mechanism(s).

 

C) Dualized-Purposed Forages with Ureolytic Ensilage with Yeast. Dual-purpose (food and feed residuals further nutrified) fodders can be nitrogen (N) supplemented via ammoniation and delignification using direct-applied protein nucleic acid (PNA) and conjugated B12 carriered gene silencing to further boost ammoniogenesis from urea (fertilizing compound) including straws (e. g. paddy straw), stovers (e. g. corn) and legume haulms (e. g. vines).  It is predicted that both convenience and sustainability will win the battle between chemical and biological approaches in feed pretreatment of this kind.

D) Particularly in certain "Cara-ranching" facilities in the Philippines there is the availability in certain regions for sugarcane tops (SCT) & bagasse pithe, and paddy straw in addition to the aquatic plant species being considered including Azolla (algae) as basal rations can be mechanized for convenience and ensiled for quality storage for year-round feeding supporting productive activities like dairy feeding. One proven e.g. is water hyacinth and paddy straw for dairy with smaller subsistence farming practices.

 

E)  Recently, we proposed at 'SkyeBlue' the launch of an investigation into functional feeding, both animal and vegetable sourced, and the role of their bioactive peptides and effect on animal biomarkers as they relate to the chronic inflammatory response and various conditions including heart health. 

 

At 'SkyeBlue' dietary treatments will be accompanied with immunomodulated biomarkered assay from blood plasma with dietary treatment of brewers-dried grains as the basal ration, with and without supplements like fishmeal or grapefruit coproduct from peel, from fruit juice manufacturing, chosen via corroborating assay and the biomarkered heart health support from peptides using these proposed functional feed components such as: 1) fishmeal (with its animal protein peptides) and 2) citrus peel (with its plant protein peptides), both candidates as dietary sources for bioactive peptides and their proposed action on immunomodulation and chronic inflammatory response in relation to health conditions such as heart health. 

 

At 'SkyeBlue' we proposed the utilization of re-entrant cannulated sheep and "sectioning" of the S. I./lower bowels (e. g. we will initially bioassay the jejunum of the S. I.) for their binding peptides at their so-called receptor sites at the inner lining and concentrating ligands there to discover their "in situ" activity after suctioned collection of the digesta fluids particularly for the digesta's soluble liquid fraction with the use of the optical fibre probe to visualize "sectioned" S. I. (at the jejunum) via our S. I. duodenal re-entrant cannulae and through an attached on-screen videocam. The biosampled lability is stopped after gut collection using solvent fixation. Then enters the application of Nobel Prize winning G. P. Winter of the U. of Cambridge from the U. K. (2018) using our proposed HPLC separation and lower gut phage display with conjoint immunoreactivity as a diagnostic to be developed using their discovery. (Phage display we hope will become a quantitatively meaningful assay for peptides with their uptake into lower gut microbiota/use of lower gut phage for display of peptides and their immunoreaction as idiotypical markers confirming their presence in the gut sectioned digesta fluid fraction, a growing area we predict with functional feeding.)