home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
  HUM-MOLGEN -> Genetic News | search  

Cost Effective Individual Genome Sequencing

  November, 1 2007 1:45
your information resource in human molecular genetics
Nature Methods presents three reports that introduce different techniques for selecting and enriching specific genomic regions in a high-throughput fashion. These methods will pave the way for cost-effective sequencing of individual human genomes, enabling large numbers of genomic regions to be extracted from a sample before sequencing and thus allowing researchers to sequence only the genomic regions of interest.

To capture in a single reaction close to 10,000 exons -- the protein encoding parts of genes -- Jay Shendure and George Church made probes identical to short-sequence stretches in the exons and used them as baits to fish out their targets. After amplification, they sequenced the captured targets with high-throughput technology.

In a complementary approach two independent groups, one led by Tom Albert and Richard Gibbs, the other by Michael Zwick, use hybridization to DNA microarrays to enrich their samples in sequences of interest. The sequences from a genome sample that are captured on the microarray can then be released and sequenced.

Author contacts:

Jay Shendure (University of Washington, Seattle, WA, USA)
E-mail: shendure@u.washington.edu

George Church (Harvard Medical School, Boston, MA, USA)

Tom Albert (NimbleGen Systems Inc, Madison, WI, USA)
E-mail: talbert@nimblegen.com

Richard Gibbs (Human Genome Sequencing Center, Houston, TX, USA)
E-mail: agibbs@bcm.tmc.edu

Michael Zwick (Emory University School of Medicine, Atlanta, GA, USA)
E-mail: mzwick@genetics.emory.edu

Abstracts available online:

Report 1.

Report 2.

Report 3.

(C) Nature Medicine press release.

Message posted by: Trevor M. D'Souza

print this article mail this article
Latest News
Variants Associated with Pediatric Allergic Disorder

Mutations in PHF6 Found in T-Cell Leukemia

Genetic Risk Variant for Urinary Bladder Cancer

Antibody Has Therapeutic Effect on Mice with ALS

Regulating P53 Activity in Cancer Cells

Anti-RNA Therapy Counters Breast Cancer Spread

Mitochondrial DNA Diversity

The Power of RNA Sequencing

‘Pro-Ageing' Therapy for Cancer?

Niche Genetics Influence Leukaemia

Molecular Biology: Clinical Promise for RNA Interference

Chemoprevention Cocktail for Colon Cancer

more news ...

Generated by News Editor 2.0 by Kai Garlipp
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 © 1995-2023 HUM-MOLGEN. All rights reserved. Liability, Copyright and Imprint.