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Nature Methods presents three reports that introduce different techniques for selecting and enriching specific genomic regions in a high-throughput fashion. These methods will pave the way for cost-effective sequencing of individual human genomes, enabling large numbers of genomic regions to be extracted from a sample before sequencing and thus allowing researchers to sequence only the genomic regions of interest.
To capture in a single reaction close to 10,000 exons -- the protein encoding parts of genes -- Jay Shendure and George Church made probes identical to short-sequence stretches in the exons and used them as baits to fish out their targets. After amplification, they sequenced the captured targets with high-throughput technology. In a complementary approach two independent groups, one led by Tom Albert and Richard Gibbs, the other by Michael Zwick, use hybridization to DNA microarrays to enrich their samples in sequences of interest. The sequences from a genome sample that are captured on the microarray can then be released and sequenced. Author contacts: Jay Shendure (University of Washington, Seattle, WA, USA)
E-mail: shendure@u.washington.edu George Church (Harvard Medical School, Boston, MA, USA)
Tom Albert (NimbleGen Systems Inc, Madison, WI, USA)
E-mail: talbert@nimblegen.com Richard Gibbs (Human Genome Sequencing Center, Houston, TX, USA)
E-mail: agibbs@bcm.tmc.edu Michael Zwick (Emory University School of Medicine, Atlanta, GA, USA)
E-mail: mzwick@genetics.emory.edu Abstracts available online: Report 1. Report 2. Report 3. (C) Nature Medicine press release.
Message posted by: Trevor M. D'Souza
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