home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
 
  HUM-MOLGEN -> Genetic News | search  
 

New Cell Source For Cloning Experiments?

 
  June, 14 2007 19:46
your information resource in human molecular genetics
 
     
Researchers may have come up with a new source of cells that could be used for nuclear transfer experiments and stem cell research. The research, reported in Nature, brings the prospect of therapeutically relevant, genetically tailored embryonic stem cell lines one step closer.

Until now, production of cloned animals and embryonic stem cell lines has relied on the use of unfertilized eggs - DNA is injected into a donor egg that has had its nucleus removed. However, Kevin Eggan and colleagues now show that fertilized eggs can also be used, suggesting that the activities required for genetic reprogramming persist after fertilization.

The team used newly fertilized mouse eggs in which cell division had been temporarily halted. The eggs' chromosomes were replaced with genetic material taken from mouse ES cells, yielding nine cloned animals. Of these, seven died from breathing difficulties, one died from a severe developmental defect and the last was rejected by its foster mother. To see if the method could be used to derive ES cells like those envisioned for patient-tailored stem cell therapies, they replaced the genetic material of fertilized mouse eggs with that of mature skin cells from mouse tails. These eggs divided to form blastocysts, and from these they were able to derive embryonic stem cells. They were also able to use defective zygotes as recipients to produce ES cells, suggesting that defective human zygotes available from IVF clinics could be used in this way.

Although the cloned animals' health is clearly a problem, suggesting that there were problems in nuclear reprogramming using this method, the study does indicate that early fertilized eggs or cells taken from them may eventually prove useful in creating patient-derived human embryonic stem cells. This is particularly important given the paucity of eggs available for cloning research and the controversy that surrounds egg donation.

CONTACT

Kevin Eggan (Harvard University, Cambridge, MA, USA)
E-mail: eggan@mcb.harvard.edu

Alan Colman (ES Cell International & A*STAR Institute of Medical Biology, Helios, Singapore)
E-mail: acolman@escellinternational.com

(C) Nature press release.


Message posted by: Trevor M. D'Souza

print this article mail this article
Bookmark and Share this page (what is this?)

Social bookmarking allows users to save and categorise a personal collection of bookmarks and share them with others. This is different to using your own browser bookmarks which are available using the menus within your web browser.

Use the links below to share this article on the social bookmarking site of your choice.

Read more about social bookmarking at Wikipedia - Social Bookmarking

Latest News
Variants Associated with Pediatric Allergic Disorder

Mutations in PHF6 Found in T-Cell Leukemia

Genetic Risk Variant for Urinary Bladder Cancer

Antibody Has Therapeutic Effect on Mice with ALS

Regulating P53 Activity in Cancer Cells

Anti-RNA Therapy Counters Breast Cancer Spread

Mitochondrial DNA Diversity

The Power of RNA Sequencing

‘Pro-Ageing' Therapy for Cancer?

Niche Genetics Influence Leukaemia

Molecular Biology: Clinical Promise for RNA Interference

Chemoprevention Cocktail for Colon Cancer

more news ...

Generated by News Editor 2.0 by Kai Garlipp
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 © 1995-2017 HUM-MOLGEN. All rights reserved. Liability, Copyright and Imprint.