home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
 
  HUM-MOLGEN -> Genetic News | search  
 

DNA Breakage In Living Color

 
  May, 31 2007 8:45
your information resource in human molecular genetics
 
     
Spontaneous breaks of replicating DNA in bacteria occur at a rate much lower than suspected, according to a study to be published online in Nature Genetics. This is the first direct measurement of DNA breakage in normal living cells, and suggests that the potential for such damage to cause larger chromosomal rearrangements is much greater than previously realized.

The cellular machinery that replicates DNA sometimes stalls. While these stalled 'replication forks' can be resolved by cleaving the DNA, which allows replication to start again, the frequency of these spontaneous breaks has been unknown. Jeanine Pennington and Susan Rosenberg devised a way to visualize individual bacteria (E. coli) with such a break by inserting a gene encoding a green fluorescent protein that would be triggered by the cell's response to DNA damage.

The authors observed that approximately one per cent of the cells in their experiment had at least one spontaneous break per generation. Previous reports based on indirect measurements had suggested a rate 20- to 100-fold higher. This low rate of spontaneous breakage suggests that each break must be a much more potent cause of the dangerous genetic instability that can result from the attempt to repair them. The authors suggest that cells, including those of higher organisms, have likely developed robust ways to avoid the creation of such DNA breaks.

Author contact:

Susan Rosenberg (Baylor College of Medicine, Houston, TX, USA)
E-mail: smr@bcm.tmc.edu

Abstract available online.

(C) Nature Genetics press release.


Message posted by: Trevor M. D'Souza

print this article mail this article
Bookmark and Share this page (what is this?)

Social bookmarking allows users to save and categorise a personal collection of bookmarks and share them with others. This is different to using your own browser bookmarks which are available using the menus within your web browser.

Use the links below to share this article on the social bookmarking site of your choice.

Read more about social bookmarking at Wikipedia - Social Bookmarking

Latest News
Variants Associated with Pediatric Allergic Disorder

Mutations in PHF6 Found in T-Cell Leukemia

Genetic Risk Variant for Urinary Bladder Cancer

Antibody Has Therapeutic Effect on Mice with ALS

Regulating P53 Activity in Cancer Cells

Anti-RNA Therapy Counters Breast Cancer Spread

Mitochondrial DNA Diversity

The Power of RNA Sequencing

‘Pro-Ageing' Therapy for Cancer?

Niche Genetics Influence Leukaemia

Molecular Biology: Clinical Promise for RNA Interference

Chemoprevention Cocktail for Colon Cancer

more news ...

Generated by News Editor 2.0 by Kai Garlipp
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 © 1995-2017 HUM-MOLGEN. All rights reserved. Liability, Copyright and Imprint.