home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
 
  HUM-MOLGEN -> Genetic News | search  
 

Approach To Target Essential Genes In C. albicans: Adaptable To Similar Pathogens

 
  March, 5 2001 10:45
your information resource in human molecular genetics
 
     
Essential antifungals

The number of infections caused by fungal organisms has increased worldwide in the past decade. Such infections can be quite serious in immunocompromised patients such as those receiving chemotherapy, undergoing organ transplants, or infected with HIV. Unfortunately, the number of antifungal drugs available to treat these infections is limited. In this issue (Nature Biotechnology, Vol. 19, No. 3, 01 Mar 2001), however, Marianne De Backer and her colleagues have identified genes critical for growth in Candida albicans, a common opportunistic pathogen, and used the genes as potential targets in an antifungal drug screen.

Until now, it has been difficult to identify novel genes in C. albicans that would be useful as targets for antifungal drugs because conventional genetic analysis is stymied by the fungus's complicated lifecycle. But using information on the C. albicans genome sequence, De Backer's team has designed a system that can theoretically suppress any of C. albicans complement of genes and test whether those genes are essential for the pathogen's growth.

By designing synthetic nucleic acids that bind to genes identified in the C. albicans genome sequence, the Belgian team were able to suppress the expression and translation of 86 genes that had a detrimental effect on growth of the fungus. Although half of these genes are of unknown function, many are known to be essential in other organisms, suggesting the approach's success. In further experiments, the researchers then engineered C. albicans mutants in several of the "essential" genes and screened them for sensitivity to antifungal drugs. The authors suggest the approach may be adaptable to other similar pathogenic organisms.

Contact:

Dr. Marianne D. De Backer
Department of Advanced Bio-Technologies
Janssen Pharmaceutica
Turnhoutseweg 30
B2341 Beerse
Belgium
Email: mdebacke@prius.jnj.com


Dr. Dominique Sanglard
Institute of Microbiology
Rue de Bugnon 44
University Hospital Lausanne
(CHUV), 1011, Lausanne
Switzerland
Email: Dominique.Sanglard@chuv.hospvd.ch

(C) Nature Biotechnology press release.


Message posted by: Trevor M. D'Souza

print this article mail this article
Bookmark and Share this page (what is this?)

Social bookmarking allows users to save and categorise a personal collection of bookmarks and share them with others. This is different to using your own browser bookmarks which are available using the menus within your web browser.

Use the links below to share this article on the social bookmarking site of your choice.

Read more about social bookmarking at Wikipedia - Social Bookmarking

Latest News
Variants Associated with Pediatric Allergic Disorder

Mutations in PHF6 Found in T-Cell Leukemia

Genetic Risk Variant for Urinary Bladder Cancer

Antibody Has Therapeutic Effect on Mice with ALS

Regulating P53 Activity in Cancer Cells

Anti-RNA Therapy Counters Breast Cancer Spread

Mitochondrial DNA Diversity

The Power of RNA Sequencing

‘Pro-Ageing' Therapy for Cancer?

Niche Genetics Influence Leukaemia

Molecular Biology: Clinical Promise for RNA Interference

Chemoprevention Cocktail for Colon Cancer

more news ...

Generated by News Editor 2.0 by Kai Garlipp
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 © 1995-2016 HUM-MOLGEN. All rights reserved. Liability, Copyright and Imprint.