home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
 
  HUM-MOLGEN -> Genetic News | search  
 

Gene Synthesis Gets Cheaper

 
  January, 11 2005 8:58
your information resource in human molecular genetics
 
     
A new method for synthesizing specific DNA sequences could revolutionize the production of genes in the laboratory (Nature, 23 Dec 04, Vol. 432, No. 7020, pp. 1050-1054). The technique, which uses programmable 'DNA microchips', looks set to slash the current cost of gene synthesis.

The DNA microchips are tagged with thousands of different short DNA sequences called oligonucleotides. These form two groups: 'construction' oligonucleotides, which act as templates for the replication of corresponding genetic sequences, and 'selection' oligonucleotides, which reinforce production of the correct sequences to minimize errors. A single-step reaction then assembles these short DNAs into much longer stretches of sequence.

To test their method, George Church and his colleagues assembled all 21 genes used by the bacterium Escherichia coli to create one part of its protein-assembly apparatus, called the ribosome. By tweaking the sequences of the construction oligonucleotides, they were able to increase the efficiency with which these genes were translated into protein, bringing closer the goal of creating a complete artificial ribosome in the lab. If successful, the technique could radically cut the cost of assembling gene sequences, which currently yields about nine DNA 'letters' for every dollar spent. The researchers hope that with this new method, a dollar could potentially buy 20,000 letters of highly accurate code.

CONTACT

George Church (Harvard University, Boston, MA, USA)
Tel: +1 617 432 7562

(C) Nature press release.


Message posted by: Trevor M. D'Souza

print this article mail this article
Bookmark and Share this page (what is this?)

Social bookmarking allows users to save and categorise a personal collection of bookmarks and share them with others. This is different to using your own browser bookmarks which are available using the menus within your web browser.

Use the links below to share this article on the social bookmarking site of your choice.

Read more about social bookmarking at Wikipedia - Social Bookmarking

Latest News
Variants Associated with Pediatric Allergic Disorder

Mutations in PHF6 Found in T-Cell Leukemia

Genetic Risk Variant for Urinary Bladder Cancer

Antibody Has Therapeutic Effect on Mice with ALS

Regulating P53 Activity in Cancer Cells

Anti-RNA Therapy Counters Breast Cancer Spread

Mitochondrial DNA Diversity

The Power of RNA Sequencing

‘Pro-Ageing' Therapy for Cancer?

Niche Genetics Influence Leukaemia

Molecular Biology: Clinical Promise for RNA Interference

Chemoprevention Cocktail for Colon Cancer

more news ...

Generated by News Editor 2.0 by Kai Garlipp
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 © 1995-2016 HUM-MOLGEN. All rights reserved. Liability, Copyright and Imprint.