The Mammalian Gene Collection page has moved to:
The NIH Mammalian Gene Collection (MGC) seeks to identify and sequence a representative full open reading frame (ORF) clone for each human and mouse gene. Over the past year the MGC has produced over 80 cDNA libraries enriched for full-length cDNAs derived from human tissue and cell lines, and mouse tissue. The quality of each library quality is first assessed by 5' and 3' EST analysis of a limited number of clones. An additional 10,000-30,000 5' EST reads are generated from each library meeting the quality standards set by the project. Several algorithms are applied to select putative full ORF clones.
As of February 2001, more than 500,000 5' EST reads have been generated, and a non-redundant set of more than 20,000 human and mouse putative full ORF clones has been assembled. These clones are subjected to high accuracy full-insert sequencing and assessed for full open reading frames. More than 3,000 clones have been fully sequenced and about 75% of the selected clones proved to have full ORFs.
All of the MGC resources, including EST sequences, full-insert sequences, and clones are fully accessible by the community.
Message posted by: Frank S. Zollmann