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Cryo-EM: Visualizing Biomolecules with High-Throughput Single Particle Analysis

 
  January 24, 2024  
     
 
Xtalks, Online
2024-03-06


The field of cryogenic electron microscopy (cryo-EM) exploded in popularity during the “resolution revolution” in the 2010s, leading to the 2017 Nobel Prize in Chemistry awarded to Jacques Dubochet, Joachim Frank and Richard Henderson “for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution.”

Cryo-EM techniques such as single particle analysis (SPA) now allow structure determination of biomolecules at resolutions before only being reached by X-ray crystallography. This technique offers many new possibilities, as it is no longer required to crystalize proteins, and allows structural determination of large protein complexes imaged in frozen solution or even in the cellular environment.

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The CRYO ARMS are equipped with a cold field emission gun for superior resolution, a 3 condenser lens stack for the CRYO ARM 200 and a 4 condenser lens stack for the CRYO ARM 300, a simple-to-operate in-column Omega energy filter and hole-free phase plates for higher contrast, maglev pumps which ensure high-vacuum and eliminate the need for frequent thawing of the instrument, an automatic and robust sample exchange system that can accommodate up to 12 samples and a long-term storage sample chamber that can be used to store and revisit samples. JEOL offers different options for sample cartridges that reduce consumable costs and can also accommodate autogrids. Finally, the CRYO ARMs offer fringe-free and come-free image shift for increased data collection throughput.

In this webinar, the featured speaker will introduce the CRYO ARM 200 and 300, describe the features that make the CRYO ARMS unique and describe workflows that allow for efficient and high-throughput structural analysis.

Join this webinar to learn about innovative approaches in cryo-EM, including high-throughput single particle analysis and more.

Keywords: Antibodies, Antibody, Drug Discovery, Proteomics, Laboratory Technology, Protein, Protein Therapeutic, Basic Research, Other Research, Electron Microscopy
 
 
Organized by: Xtalks
Invited Speakers: Emmanuel Smith, Senior Application Specialist for Cryo-EM, JEOL USA
 
Deadline for Abstracts: 2024-03-06
 
Registration: Free Registration
E-mail: tristan@xtalks.com
 
 
   
 
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