Intestinal Organoids As a Model System and Tools for Genetic Manipulation

Organoids (primary 3D epithelial culture) can be derived from many mouse and human tissues. This novel culture system has enabled us to explore gene functions in many diverse types of primary cells.

In this webinar, the murine intestinal organoid will be introduced as a novel model system.  Several genetic manipulation tools for organoids will then be discussed with examples, including retroviral transgenesis, BAC-transgenesis, and CRISPR/Cas gene editing.

This webinar will review:

• • Intestinal 3D organoid culture system
  • Genetic manipulation tools for organoids

 Register today to hear more about this exciting research!

Dr Bon-Kyoung Koo 

Dr. Bon-Kyoung Koo is an experienced mouse geneticist actively employing the emerging organoid culture technology to study stem cell maintenance and activation in mouse and human intestinal and stomach organoid cultures.

Following his PhD, Dr. Koo joined the group of Prof. Hans Clevers at the Hubrecht Institute, KNAW, Netherlands. Here he utilised novel intestinal organoid culture system to perform in vitro genetics.

Dr. Koo is currently a principal investigator at the Cambridge Stem Cell Institute at the University of Cambridge, where he is developing novel genetic tools to further investigate how proliferating and quiescent adult epithelial stem cell populations are regulated both in homeostasis and regeneration.