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Advanced Techniques in Molecular Neuroscience

  December 29, 2017  

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
June 29 - July 14, 2018

This laboratory and lecture course is designed to provide neuroscientists at all levels with a conceptual and practical understanding of several of the most advanced techniques in molecular neuroscience. The course curriculum is divided into three sections: an extensive and up-to-date set of laboratory exercises, daily lectures covering the theoretical and practical aspects of the various methods used in the laboratory, and a series of evening research seminars. The informal and interactive evening lectures will be given by leading molecular neuroscientists and will serve to illustrate the ways in which the various experimental approaches have been used to advance specific areas of neurobiology. In this year's course, the laboratory portion will include topics such as: an introduction to the design and use of animal virus vectors in neurobiology; the use of CRISPR genome editing and RNAi approaches for regulating the expression of specific genes in neurons; practical exercises in gene delivery systems including mammalian cell infection and transfection and electroporation techniques for targeted gene transfer in vivo; an introduction to overall strategies, use and design of BAC transgenic vectors; real time RT-PCR analyses; assays of chromatin and chromatin structure in neurons, and mRNA isolation from specified neural subtypes by TRAP.
Organized by: Instructors: Cary Lai, Joseph LoTurco, Anne Schaefer
Invited Speakers: TBA
Deadline for Abstracts: March 31 2018
Registration: Apply here 
E-mail: vuong@cshl.edu
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