The Advanced Bacterial Genetics course presents logic and methods used in the genetic dissection of complex biological processes in diverse bacteria. Laboratory methods include: classical and cutting-edge mutagenesis using transposons, allelic exchange, and TargeTron; recombineering with single and double stranded DNA; CRISPR/Cas genome editing; genome sequencing and assembly; mapping mutations using genetic and physical techniques; modern approaches to the generation and analysis of targeted gene disruptions and reporter gene fusions; fluorescence microscopy. Key components of the course will be the use of sophisticated genetic methods in the analysis of model bacteria (including Escherichia coli, Salmonella, Staphylococcus aureus, and Vibrio cholerae), and the use of the wealth of new genomic sequence information to motivate these methods.
Invited lecturers will present various genetic approaches to study bacterial mechanisms of metabolism, development, and pathogenesis.
The course admits 16 students, both foreign and US residents, from diverse backgrounds and career levels for intensive (but fun) instruction in microbial genetics.
For further academic information about the course, feel free to contact the instructors.