EMBL Course: Next Generation Sequencing: Amplicon Based Targeted Resequencing

The goal of this course is to teach the fundamentals of NGS library preparation and validation using an Amplicon PCR based test like the TruSeq Amplicon assay. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the wet lab preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quality control and normalize the prepared libraries. We will also have a basic training on assay design using DesignStudio and data analysis of TruSeq Amplicon sequencing results using standard Illumina tools like MiSeq Reporter and VariantStudio.