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ION CHANNELS & SYNAPTIC TRANSMISSION

 
  January 18, 2013  
     
 


Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
June 5 - 25, 2013


The primary goal of this course is to investigate, through lectures and laboratory work, the properties of ion channels that allow neurons to carry out their unique physiological functions in a variety of neural systems. Areas of particular interest include channels that (1) are activated by neurotransmitter at central and peripheral synapses, (2) are activated by voltage changes in axons and dendrites, (3) respond to neuromodulators with changes in functional properties, (4) are developmentally required and regulated, or (5) are light-gated and engineered to express in specific neural subtypes. The research interests of guest lecturers reflect these areas of emphasis.

The laboratory component of the course introduces students to state-of-the-art electrophysiological approaches for the study of ion channels in their native environments. Hands-on exercises include patch-clamp recording of ion channel activity in acutely isolated or cultured cells or neurons in brain slice preparations. Different recording configurations are used (e.g. whole-cell, cell-attached, dendritic patches, and using voltage- and current-clamp configurations) to examine macroscopic or single-channel activity. Similarly, various methods of ligand and drug application are demonstrated. The advantages and disadvantages of each method, preparation and recording technique are considered in relation to the specific scientific questions being asked. Admissions priority is given to students and postdocs with a demonstrated interest, specific plans, and a supportive environment to apply these techniques to a defined problem.

 
 
Organized by: Stephan Brenowitz, Ian Duguid, Paul Kammermeier
Invited Speakers:

TBA
 
Deadline for Abstracts: March 15, 2013
 
Registration: Apply here
E-mail: meetings@cshl.edu
 
   
 
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