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ADVANCED BACTERIAL GENETICS

 
  January 18, 2013  
     
 


Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
June 5 - 25, 2013


The Advanced Bacterial Genetics course presents logic and methods used in the genetic dissection of complex biological processes in diverse bacteria. Laboratory methods include: classical mutagenesis using transposons, mutator strains, and chemical mutagens; recombineering with single and double stranded DNA; detection of gene expression changes using various reporter genes; the mapping of mutations using genetic and physical techniques; modern approaches to the generation and analysis of targeted gene disruptions and fusions using PCR and cloning methods; epitope insertion mutagenesis; site-directed mutagenesis; and fluorescence microscopy. Key components of the course will be the use of sophisticated genetic methods in the analysis of model bacteria (including Escherichia coli, Salmonella, Bacillus subtilis, and Vibrio cholerae), and the use of the wealth of new genomic sequence information to motivate these methods.

 
 
Organized by: Diarmaid Hughes, Beth Lazazzera, Fitnat Yildiz
Invited Speakers:

TBA
 
Deadline for Abstracts: March 15, 2013
 
Registration: Apply here
E-mail: meetings@cshl.edu
 
   
 
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