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PharmaTraining Limited, Window Conference Venue, 13 Windsor Street. Islington, London, N1 8QG
25 & 26 September 2012
The course will commence with registration and coffee at 8.30, course proper starts at 9.00 and will finish at 17.00
Day 1
09:00 Introduction. Introduction to LC–MS. System overview. Data-acquisition and processing. Role of LC–MS in quantitative bioanalysis.
10:15 Coffee break
10:30 Analyte ionisation for LC–MS: electrospray and atmospheric-pressure chemical ionisation. Instrumentation, chemistry, ionisation mechanism, flow-rates, mobile-phase composition. Introduction to matrix effects.
12:30 Lunch break
13:30 Information from LC–MS mass spectra. The analyte mass spectrum is a diagnostic tool. One should learn to appreciate the information that is present in the spectrum, e.g., adductions and adduct-bound dimers, but also topics related to average and monoisotopic mass, mass defects, and isotope peaks.
15:00 Coffee break
15:15 Workflow in quantitative analysis using LC–MS: Sample pretreatment, liquid chromatography, analyte ionisation, mass spectrometry, data processing.
17:00 End of the first day.
Day 2
09:00 Method development - 1: General aspects. Selected reaction monitoring (SRM). Selection of transitions. Multiresidue methods. Selection of an appropriate internal standard. General method development.
10:15 Coffee break
10:30 Method development - 2: Matrix effects. Methods to evaluate sample pre-treatment and separation methods with respect to matrix effects.
12:30 Lunch
13:50 Method development - 3: Sample pre-treatment methods and liquid chromatography aspects for bioanalysis. Directives for method development.
15:00 Coffee break
15:15 Selected applications. Validation and troubleshooting: Recognition of problems, detection and solution to problems.
17:00 End of the second day and end of the course.
Quantitative Bioanalysis using LC-MS for Pharma
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Organized by:
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PharmaTraining Ltd |
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Invited Speakers:
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Wilfried Niessen has almost 30 years of experience as a researcher and project manager in the field of analytical mass spectrometry, and especially liquid chromatography – mass spectrometry. Since 1996, he works as an independent consultant, providing expert consultancy and (advanced) courses in analytical mass spectrometry.. William van Dongen holds a PhD (1996) in peptide and protein mass spectrometry. Since then, he has worked as an industrial researcher, project manager and laboratory manager in the field of bioanalytical mass spectrometry and liquid chromatography – mass spectrometry. He was responsible for setting up the bioanalytical LC–MS facility of Pharma io-Research (currently PRA International), one of the first contract laboratories offering commercial LC–MS services to the pharma industry. He is (co-) author of 25 refereed papers in the field of protein and peptide mass
spectrometry and bioanalytical LC–MS.
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Deadline for Abstracts:
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n/a
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Registration:
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Full Fee: 2 day course £1180.00 (+ VAT £236.00 if applicable, see VAT NOTES))
Discounted rate of 10% for booking 8 weeks in advance (24 July 2012)
Discounted rate of 10% for booking more than 1 delegate
Discounted rate of 10% for booking more than 1 course
Maximum discount received is 15%
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E-mail:
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judy@pharma-training-courses.com
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