Euroscicon, London, UK
17 October 2012
Meeting Chair: Dr Will Howat, Head of Histopathology/ISH at Cambridge Research Institute, Cancer Research UK This event has CPD accreditation On registration please submit your questions to the panel that will be asked by the chair on the day of the event 9:00 – 9:30 Registration 9:30 – 9:35 Introduction by Meeting Coordinator: Dr Astrid Englezou , Euroscicon, London, UK 9:35 – 9:40 Introduction by the Chair: Dr Will Howat, Head of Histopathology/ISH at Cambridge Research Institute, Cancer Research UK 9:35– 9:55 Title to be confirmed
Dr Will Howat, Head of Histopathology/ISH at Cambridge Research Institute, Cancer Research UK
9:55 – 10:15 Immunohistochemistry for Glycol Methacrylate embedded samples
Dr Susan J Wilson PhD, Head of the Histochemistry Research Unit, Southampton General Hospital
Glycol methacrylate (GMA) was introduced in the 1960s and is a hydrophilic acrylic resin. By using a modified processing and embedding procedure it can be used for immunohistochemical studies. This offers several advantages over traditional techniques including good antigen preservation, excellent morphology and antigen localisation, no need for antigen retrieval, cutting of numerous serial sections is possible and co-localisation studies. We use an avidin-biotin peroxidase detection system and have applied this methodology to a wide range of samples including lung, gut, kidney, skin, conjunctiva, tissue culture monolayers and dispersed cells. Details of the technique including fixation, processing, sectioning and the immunohistochemical protocol will be discussed. 10:15 – 10:35 Talk to be confirmed 10:35 – 10:55 Title to be confirmed
Urban Ryberg, The Human Protein Atlas project, Uppsala, Sweden 10:55 – 11:25 Mid-morning Break Please try to visit all the exhibition stands during your day at this event. Not only do our sponsors enable Euroscicon to keep the registration fees competitive, but they are also here specifically to talk to you 11:25 – 11:45 Making the most of tissue microarrays
Dr Anthony Warford, Warford Technology Ltd, UK
Tissue microarrays (TMAs) have revolutionised the way in which research is done in cellular pathology. In essence, they miniaturise the amount of tissue and time required to generate statistically meaningful results. To achieve this, TMAs must be prepared carefully. First and foremost, tissue cores from each donor sample must be representative of the histology or pathology. To achieve this guided tissue selection must be used and consideration given to the diameter and number of cores removed from each donor sample. Led by the research question to be answered the number of donor samples in each TMA should also be carefully considered. Properly made hundreds of sections can be cut from a TMA block and these should be protected from antigen degradation before staining. Lastly, once staining has been completed, analysis should be undertaken in a consistent manner to maximise the return on the investment in TMA preparation. 11:45 – 12:05 Talk to be confirmed 12:05 – 12:40 Working Lunch Please collect your lunch and take it to your discussion table (Session 1) This is also a good time to fill out your feedback forms 12:40 – 14:10 Discussion Group Sessions 1 - 3 - Round table discussion groups (20 minutes each) will be held throughout the afternoon
- Delegates will rotate so that they may participate in all the discussion tables
- All delegates will also be allocated a session for visiting the exhibition stands
- Where appropriate delegates will be able to bring their samples to the discussions
- See end of agenda for description of discussion tables
14:10 – 14:30 Tissue Cross-reactivity Studies: current considerations
Joanne Mitchell, Huntingdon Life Sciences, Huntingdon, UK 14:30 – 15:35 Discussion Group Sessions 4 - 6 15:35 – 16:20 Question and Answer Session This session will include summing up of the discussion tables and questions submitted both prior to the meeting and throughout the day 16:20 – 16:30 Chairman’s Summing Up and Feedback Prize Draw
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