Center for Professional Innovation & Education, Inc., Los Angeles, CA
Nov. 12 – 14, 2012
There is an increasing demand for large quantities of human and/or other mammalian proteins to support drug discovery and development efforts. These proteins are not only valued in the drug screening/discovery process but are now becoming increasingly important as direct therapeutic agents. As most therapeutically relevant proteins are only expressed in small quantities in their natural environment, the need for expression of these proteins at high levels has become increasingly important. This course will describe the strategies and techniques one can utilize to maximize protein expression in heterologous systems. The latter systems include E.coli, baculovirus, mammalian and alternative hosts. Specific examples and case studies will be given to demonstrate the capabilities and limitations of each platform Participants will learn how to choose an appropriate host system, the best choice of vector and cell lines, and the fundamental technology needed to implement the platforms in their own laboratories. Those already familiar with these platforms will be able to benefit as new strategies will be presented to familiarize them with improved expression activities and troubleshooting techniques.
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