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Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
July 24- August 13, 2012
Advances in light microscopy, digital image processing, and the development of a variety of powerful fluorescent probes present expanding opportunities for investigating the nervous system, from synaptic spines to networks in the brain. This intensive laboratory and lecture course will provide participants with the theoretical and practical knowledge to utilize emerging imaging technologies. The primary emphasis of the course will be on vital light microscopy. Students will learn the principles of light microscopy, as well as use of different types of electronic cameras, laser scanning systems, functional fluorophores, delivery techniques, and digital image-processing software. In addition to transmitted light microscopy for viewing cellular motility, the course will examine a variety of molecular probes of cell function, including calcium-sensitive dyes, voltage-sensitive dyes, photo-activated ("caged") compounds, and exocytosis tracers. Issues arising in the combination of imaging with electrophysiological methods will be covered. Particular weight will be given to multi-photon laser scanning microscopy and to newly available biological fluorophores, especially Green-Fluorescent Protein (GFP) and its variants. We will use a spectrum of neural and cell biological systems, including living animals, brain slices, and cultured cells.
Applicants should have a strong background in the neurosciences or in cell biology. In their personal statements, applicants should specify: 1) their experience with optical techniques, 2) how they will apply optical methods in their current projects, 3) the microscope systems available to them, and 4) their long-term goals in learning more about optical methods.
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Organized by:
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David DiGregorio, Jack Waters |
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Invited Speakers:
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Lecturers in the 2011 course included:
Florin Albeanu, Cold Spring Harbor Laboratory
Thomas Blanpied, University of Maryland School of Medicine
Marcel Bruchez, Carnegie Mellon University
Karl Deisseroth, Stanford University
Winfried Denk, Max-Planck-Institute for Medial Research
Jeremy Dittman, Weill Cornell Medical College
Christian Eggeling, Max Planck Institute forBiophysical Chemistry
Valentina Emiliani, CNRS, INSERM, University Paris Descartes
Florian Engert, Harvard
Nicolas George, Olympus America Inc.
Oliver Griesbeck, Max Planck Institute of Neurobiology
Mark Hubener, Max-Planck-Institute of Neurobiology
Fred Lanni, Carnegie-Mellon University
Lowell (Butch) Moomaw, Hamamatsu Photonic Systems
Thomas Mrsic-Flogel, University College London
Venkatesh Murthy, Harvard University
Darcy Peterka, HHMI and Columbia University
Kate Pritchard, Hamamatsu Photonic Systems
Philbert Tsai, University of California, San Diego
William Tyler, Virginia Tech Carilion School of Medicine & Research Institute
Steven Vogel, NIH/NIAAA/DICBR
Keith Weninger, North Carolina State University
Linda Wilbrecht, UCSF
Visiting faculty will change from year to year, and the specific topics covered will vary. Potential areas include:
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Deadline for Abstracts:
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April 15, 2012
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Registration:
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http://meetings.cshl.edu/course/Default.aspx
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E-mail:
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meetings@cshl.edu
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