Wellcome Trust Advanced Courses, Wellcome Trust Genome Campus, Hinxton, UK
15-24 April 2012
This new course provides a comprehensive overview of exome sequencing, also known as pulldown, sequence capture and targeted sequencing.
Participants will gain practical laboratory experience as well as training in downstream data analysis, interpretation and validation.
Solution hybrid capture is used to enrich for all known exon sequences (the exome) or for customised regions from genomic DNA for the purpose of sequencing the regions of interest.The enriched targets are sequenced using next generation technology to a high coverage and analysed for variants.
Material covered will include:
sample quality assessment and logistics
DNA fragmentation and quantification
DNA library preparation
overview of Illumina sequencing
SNP consequence analysis
protein impact analysis
rare SNP discovery
copy number variation.
Teaching will be complemented by opportunities to interact with academic and technical experts working at the cutting edge of the field.