PharmaTraining Limited, Window Conference Venue, 13 Windsor Street, Islington, London, N1 8QG, United Kingdom
2011-09-19
Overview This one-day course provides an introduction to technology and (potential) application areas of combined liquid chromatography-mass spectrometry (LC–MS) in small-molecule pharmaceutical industry. The morning sessions provide a clear technology overview, introducing analyte ionization techniques, different types of mass analysers, and important data acquisition strategies. The two afternoon sessions focus on applications in quantitative and qualitative analysis, respectively. Course Programme Course will commence with registration and coffee at 8.30, course proper starts at 9.00 and will finish at 5.00 09:00 Introduction. Identifying the pharmaceutical application areas for LC–MS. Principles of LC–MS and LC–MS–MS. Data acquisition strategies in LC–MS.
10:30 Coffee break
10:45 Solutions by LC–MS: Instrumentation overview. Electrospray and APCI. Flow-rate and mobile-phase composition. Different types of mass analysers: how and when about single quadrupole, triple quadrupole, iontrap, and time-of-flight instruments.
12:30 Lunch
13:30 Potential and limitations of LC–MS in quantitative (bio)analysis. General workflow in quantitative analysis. Selected reaction monitoring. Analytical strategies involved in bioavailability, PK/PD studies, metabolic stability, genotoxic impurities. Validation issues.
15:15 Coffee break
15:30 LC–MS in qualitative applications. Fragmentation in MS–MS as a tool for structure elucidation, Multistage MSn and accurate-mass determination. Software tools. Applications in checking compound identity, impurity
profiling, and metabolite identification.
17:00 End of the course
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Invited Speakers:
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Wilfried Niessen has almost 30 years of experience as a researcher and
project manager in the field of analytical mass spectrometry, and especially
liquid chromatography – mass spectrometry. Since 1996, he works as an
independent consultant, providing expert consultancy and (advanced)
courses in analytical mass spectrometry. In 2002, he was appointed
(part-time) extraordinary professor in bioanalytical mass spectrometry at
the Faculty of Science of the VU University in Amsterdam, where he is
currently interim Head of the Division BioMolecular Analysis. William van Dongen holds a PhD (1996) in peptide and protein mass
spectrometry. Since then, he worked as an industrial researcher, project
manager and laboratory manager in the field of bioanalytical mass
spectrometry and liquid chromatography – mass spectrometry. He was
responsible for setting up the bioanalytical LC–MS facility of Pharma
Bio-Research (currently PRA International), one of the first contract
laboratories offering commercial LC–MS services to the pharma industry.
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