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Quantitative Bioanalysis using LC-MS for Pharma Industry

 
  February 15, 2011  
     
 
PharmaTraining Limited, Window Conference Venue, 13 Windsor Street, Islington, London, N1 8QG, United Kingdom
2011-09-20


Overview

This advanced application-oriented two-day course in LC-MS pays attention to the
important aspects of quantitative bioanalysis by LC-MS, except for statistical aspects in
data-processing. Starting from providing knowledge on important processes in analyte
ionization by electrospray or APCI, the course provides a profound understanding of the
important topics of quantitative bioanalysis using LC-MS. Quality control in instrument
performance, method development, and systematic troubleshooting are key issues
throughout the course. The theoretical parts are illustrated with a number of case
studies. Experience with the LC-MS instrumentation is recommended prior to attending
this course The aim is to provide the participants with the general principles, background
knowledge, and how to use this knowledge in practice.

Course Programme (2 day)

The course will commence with registration and coffee at 8.30, course proper starts at 9.00 and will finish at 17.00

Day 1

09:00 Introduction. Introduction to LC–MS. System overview. Dataacquisition and processing. Role of LC–MS in quantitative bioanalysis.

10:15 Coffee break

10:30 Analyte ionisation for LC–MS: electrospray and atmospheric-pressure chemical ionisation. Instrumentation, chemistry, ionisation mechanism, flow-rates, mobile-phase composition. Introduction to matrix effects.

12:30 Lunch break

13:30 Information from LC–MS mass spectra. The analyte mass spectrum is a diagnostic tool. One should learn to appreciate the information that is present in the spectrum, e.g., adductions and adduct-bound dimers, but also topics related to average and monoisotopic mass, mass
defects, and isotope peaks.

15:00 Coffee break

15:15 Workflow in quantitative analysis using LC–MS: Sample pretreatment, liquid chromatography, analyte ionisation, mass spectrometry, data processing.

17:00 End of the first day.

Day 2

09:00 Method development - 1: General aspects. Selected reaction monitoring (SRM). Selection of transitions. Multiresidue methods. Selection of an appropriate internal standard. General method development.

10:15 Coffee break

10:30 Method development - 2: Matrix effects. Methods to evaluate sample pre-treatment and separation methods with respect to matrix effects.

12:30 Lunch

13:50 Method development - 3: Sample pre-treatment methods and liquid chromatography aspects for bioanalysis. Directives for method development.

15:00 Coffee break

15:15 Selected applications. Validation and troubleshooting: Recognition of problems, detection and solution to problems.

17:00 End of the second day and end of the course.

 
 
Organized by: PharmaTraining Limited
Invited Speakers:

Wilfried Niessen has almost 30 years of experience as a researcher and project manager in the field of analytical mass spectrometry, and especially liquid chromatography – mass spectrometry. Since 1996, he works as an independent consultant, providing expert consultancy and (advanced) courses in analytical mass spectrometry. In 2002, he was appointed (part-time) extraordinary professor in bioanalytical mass spectrometry at the Faculty of Science of the VU University in Amsterdam, where he is currently interim Head of the Division BioMolecular Analysis.

William van Dongen holds a PhD (1996) in peptide and protein mass spectrometry. Since then, he has worked as an industrial researcher, project manager and laboratory manager in the field of bioanalytical mass spectrometry and liquid chromatography – mass spectrometry. He was responsible for setting up the bioanalytical LC–MS facility of Pharma Bio-Research (currently PRA International), one of the first contract laboratories offering commercial LC–MS services to the pharma industry. He has almost ten years experience as study director of bioanalytical LC–MS studies. He started up the mass spectrometry facilities for the generic pharmaceutical company Synthon and for his current employer PROXY, a pharma contract laboratory.

 

 
Deadline for Abstracts: .
 
Registration: http://pharma-training-courses.com/Quantitative-bioanalysis-using-LC-MS-for-pharma-industry.html
E-mail: judy@pharma-training-courses.com
 
   
 
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