European Molecular Biology Laboratory, Heidelberg, Germany
2011-03-27
The goal of this course is to give an introduction to a broad range of state-of-the-art chemical biology methods and to provide a forum for active cross-communication. We will encourage the participants to apply methods of this interdisciplinary field to answer their biological questions. Emphasis will be on chemical protein engineering methods, proteomics methods, screening techniques and in vivo protein labelling as well as enzyme activity probes. Thereby, emphasis will be on (commercial) availability of the methods and reagents and on understanding of the chemical methods. Participants will do hands-on experiments supervised by experts in the field and will have plenty of time to discuss their own research problems with teachers and invited speakers. Topics that will be covered include: -
In vivo protein labeling via Snap- and Clip-tag technology -
Development and application of nucleic acid probes for detection of RNA in cells -
Photocontrollable and small-molecule-switchable enzymes -
Miniaturized lipid arrays for screening of protein-lipid interactions -
Mass spectrometry and liquid chromatography for the identification of proteins and posttranslational modifications -
Introduction of unnatural amino acids via amber suppression for site-specific protein labeling -
Expressed Protein Ligation and native chemical ligation -
Assay development and validation for high throughput screening in microtiter plate format -
Microfluidic technologies for single-cell/single organism assays and high throughput screens
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Invited Speakers:
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Confirmed Speakers Virginia Cornish, Columbia University, USA
Michael Famulok, University of Bonn, Germany
Hiroshi Sugiyama, Kyoto University, Japan
Derek Tan, Memorial Sloan–Kettering Cancer Center, USA
Teachers Anne-Claude Gavin, EMBL Heidelberg, Germany
Kai Johnsson, EPFL, Switzerland
Maja Köhn, EMBL Heidelberg, Germany
Jeroen Krijgsveld, EMBL Heidelberg, Germany
Edward Lemke, EMBL Heidelberg, Germany
Joe Lewis, EMBL Heidelberg, Germany
Christoph Merten, EMBL Heidelberg, Germany
Carsten Schultz, EMBL Heidelberg, Germany
Oliver Seitz, Humboldt University, Germany
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