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CfPA - The Center for Professional Advancement, 90 Minute Accredited Online Training
March 25, 2010 at 11:00 a.m.–12:30 p.m. (ET)
Who Should Attend
This online training will be most useful for people with little or no experience with using HPLC (High Performance Liquid Chromatography). This course will benefit laboratory personnel in many industries including: pharmaceutical – traditional and phytochemical, food, beverage, environmental, chemical, and personal products.
The potential job functions begin with new laboratory technicians to researchers with degrees in other disciplines who need to begin their own HPLC work. This includes: organic synthesis, quality control, and methods developments chemists and various research personnel.
The departments in companies involved with the use of HPLC in research/development or quality control/quality assurance should insist their new personnel attend if they are not being trained in-house. Description
This basic HPLC course will benefit the new or recent users of this analytical tool. This 90-minute accredited training will include discussions of theory, definitions, and typical practice and will illustrate what HPLC is all about. What is inside the 'skin' of the LC system will give an understanding of its basic simplicity. The course is designed to give the new user confidence in his/her use of this invaluable analytical tool.
Module 1:
• Principle & basic theory of HPLC including modes of
chromatography
• The chromatogram and nomenclature
• The vanDeemter plot and its significance
• Monitoring the LC separation
• Use of the resolution equation to aid in optimizing the LC
separation
• Peak shape, deterioration of the separation
• HPLC instrumentation, solvent reservoirs, pumps
• Mobile phase - isocratic and gradient operation
Module 2:
• Correct connections throughout the LC system
• Injectors, laminar flow in empty tubing, autoinjectors
• HPLC hardware, sizes, flow adjustments with different
diameter column
• Detectors used in HPLC - the usual and the specific
• Calibration curves for quantitative analysis
• Column packings, silica gel development, bonding
possibilities
• Bonding terminology - endcapping, base deactivated, polar
modified
• Solvent considerations, HPLC grade water, degassing
mobile phases
Module 3:
• Reversed phase mode, USP column classification
• RP column selection, other RP bonded phases to consider
• Retention differences with % carbon loading and RP chain
length
• Comparing different C18 phases
• Controlling pH and why, solvent choices for optimization
• Ion pairing to retain compounds with strong ionization
• Caution with buffer gradient separations
• LC columns out of the box, and their storage
• Application help from the web and texts
Questions and Answer Session
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Organized by:
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CfPA - The Center for Professional Advancement |
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Invited Speakers:
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Dr. Fred Rabel, President, ChromHELP, LLC
Dr. Fred Rabel, has been teaching chromatography courses for over 35 years. He is president of his company, ChromHELP, LLC. He continues to write, consult, and train chromatographers throughout the US and Canada. Dr. Rabel is on the editorial boards of Journal of Liquid Chromatography and Related Techniques and the Journal of Chromatographic Science and has published many articles and chapters on liquid chromatography.
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Deadline for Abstracts:
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n/a
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Registration:
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Please click here for registration information.
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E-mail:
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sberg@cfpa.com
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