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Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory
October 11 - 26, 2010
Crystallography and X-ray diffraction yield a wealth of structural information unobtainable through other methods. This intensified laboratory/computational course focuses on the major techniques used to determine the three-dimensional structures of macromolecules. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography. Topics to be covered include basic diffraction theory, crystallization (proteins, nucleic acids and complexes), crystal characterization, X-ray sources and optics, synchrotrons, crystal freezing, data collection, data reduction, multiple isomorphous replacement, multiwavelength anomalous diffraction, molecular replacement, solvent flattening, non-crystallographic symmetry averaging, electron density interpretation, molecular graphics, structure refinement, structure validation, coordinate deposition and structure presentation. Participants learn through extensive hands-on experiments. One or more proteins are crystallized and the structure(s) determined by several methods, in parallel with lectures on the theory and informal discussions behind the techniques. Applicants should be familiar with the creation and editing of simple text files on Linux workstations using a screen based editor (either vi or emacs).
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Organized by:
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William Furey, Gary Gilliland, Alexander McPherson, James Pflugrath |
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Invited Speakers:
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Speakers in the 2009 course included:
Paul Adams, Lawrence Berkeley Laboratory
Aina Cohen, Stanford Synchrotron Radiation Laboratory
Paul Emsley, University of Oxford, UK
Wayne Hendrickson, Columbia University
Leemor Joshua-Tor, Cold Spring Harbor Laboratory
Gerard Kleywegt, EMBL European Bioinformatics Institute, UK
Anastassis (Tassos) Perrakis, Netherlands Cancer Institute
Randy Read, University of Cambridge, UK
David Richardson, Duke University Medical Center
Jane Richardson, Duke University Medical Center
Robert Sweet, BNL
Thomas Terwilliger, Los Alamos National Laboratory
Dale Tronrud, University of Oregon
John Westbrook, Rutgers - The State University of New Jersey
Michael Wiener, University of Virginia
Joanna Yeh, University of Pittsburgh
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Deadline for Abstracts:
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June 15, 2010
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Registration:
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To Apply: http://meetings.cshl.edu/course/courseapp_instr.shtml
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E-mail:
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stephens@cshl.edu
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