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Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory
July 22 - August 4, 2010
This intensive laboratory and lecture course will focus on cutting-edge proteomics approaches and technologies. In the protein profiling portion of the course, students will gain hands-on experience in several quantitative proteome analysis methods, including two-dimensional gel electrophoresis and isotopic labeling strategies. Students will be trained to use DIGE, or differential in-gel electrophoresis, for gel-based protein quantification. Differentially expressed proteins will be determined using advanced gel analysis software, and identified using MALDI mass spectrometry. Students will be taught differential and quantitative mass spectrometry approaches to profile and identify changes in proteomes. Students will be trained in high-sensitivity microcapillary liquid chromatography coupled with nanospray-ESI and tandem mass spectrometry. Both single dimension and multidimensional microcapillary liquid chromatography separations coupled to mass spectrometry will be taught. Students will gain hands-on experience purifying and identifying protein complexes and post-translational modifications using the latest technologies. A strong emphasis will be placed on data analysis throughout the course. The overall aim of the course is to provide each student with the fundamental knowledge and hands-on experience necessary to be able to perform and analyze proteomics experiments, and to learn to identify new opportunities in applying proteomics approaches to his/her own research.
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Organized by:
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Tracy Andacht, James E. Bruce, Andrew Link, Darryl Pappin |
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Invited Speakers:
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2010 Speakers To be Announced Speakers in the 2009 course included:
Susan Abbatiello, The Broad Institute of MIT and Harvard
Anna Arnaudo, Princeton University
Brian Chait, The Rockefeller University
Pierre Chaurand, Vanderbilt University School of Medicine
Karl Clauser, Broad Institute of MIT and Harvard
Craig Dufresne, Thermo Fisher Scientific
Jimmy Eng, University of Washington
David Friedman, Vanderbilt University
Neil Kelleher, University of Illinois
Tuo Li, Princeton University
Scott Peterman, Thermofisher
Heng Zhu, Johns Hopkins University
Visiting faculty will change from year to year
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Deadline for Abstracts:
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Application Deadline is March 15, 2010
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Registration:
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http://meetings.cshl.edu/course/courseapp_instr.shtml
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E-mail:
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stephens@cshl.edu
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