Ion Channel Physiology

The primary goal of this course is to investigate, through lectures and laboratory work, the properties of ion channels that allow neurons to carry out their unique physiological functions in a variety of neural systems. Areas of particular interest include channels that (1) are activated by neurotransmitter at central and peripheral synapses, (2) are activated by voltage changes in axons and dendrites, (3) respond to neuromodulators with changes in functional properties, or (4) are developmentally required and regulated. The research interests of guest lecturers reflect these areas of emphasis.

The laboratory component of the course introduces students to electrophysiological approaches for the study of ion channels in their native environments. Hands-on exercises include patch-clamp recording of ion channel activity in acutely isolated or cultured cells or neurons in brain slice preparations. Different recording configurations are used (e.g. whole-cell, cell-free, and nucleated patches) to examine macroscopic or single-channel activity. Similarly, various methods of ligand and drug application are demonstrated. The advantages and disadvantages of each method, preparation and recording technique are considered in relation to the specific scientific questions being asked. Admissions priority is given to students and postdocs with a demonstrated interest, specific plans, and a supportive environment to apply these techniques to a defined problem.

2010 Speakers To be Announced

Guest speakers in 2009 included:
Clay Armstrong, University of Pennsylvania
Joshua Dudman, HHMI/Janelia Farm Research Campus
Michael Hausser, University College, London UK
Bertil Hille, University of Washington
Matthew Jones, University of Wisconsin, Madison
Peter Larsson, University of Miami
Massimo Mantegazza, Instituto Neurologico Besta, Itala
Massimo Scanziani, University of California, San Diego
Andreas Schaefer, Max Planck Institute, Germany
Jasper Sjostrom, University College London, UK
Ivan Soltesz, University of California, Irvine
Stephen Traynelis, Emory University
Henrique Von Gerldorff, Oregon Health & Science University