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Quantitative Fluorescent Microscopy

 
  November 06, 2009  
     
 
Mount Desert Island Biological Laboratory, Salisbury Cove, ME 04672
June 12-18, 2010


June 13, 2010
9:00-9:30 am Introduction
Welcome, Introductions, Define Course Goals
Watkins/Piston
9:30-10:30 am The Microscope 1
From Leuwenhoek to Digital Imaging
Watkins
10:30-11:00 am Break
11:00-12:00 pm The Microscope 2
Transmitted Light Contrast in Microscopy
Piston
12:00-1:00 pm Lunch
1:00-2:30 pm LAB
TRANSMITTED LIGHT MICROSCOPY
2:30-3:00 pm Break
3:00-3:30 pm The Microscope 3
Principal of Fluorescence
Piston
3:30-4:30 pm The Microscope 4
Fluorescence Microscope Design
Piston
4:30-5:00 pm Break
5:00-5:30 pm The Microscope 5
Optical Filters for Fluorescence Microscopy
Stanley
5:30-6:00 pm Digital Imaging 1
Digital Imaging and Detector Systems
Watkins
6:00-7:00 pm Dinner
7:00-8:00 pm LAB
SIGNAL-TO-NOISE IN DIGITAL IMAGING
8:00-11:00 pm LAB
STANDARD FLUORESCENCE MICROSCOPY
June 14, 2010
9:00-9:30 am Digital Imaging 2
Resolution in Digital Imaging
Watkins
9:30-10:00 am Confocal 1
Introduction to Confocal Microscopes
St. Croix
10:00-10:30 am Confocal 2
Confocal Detectors and "Pseudo-confocals"
Piston
10:30-11:00 pm Break
11:00-12:00 am Live Cell Imaging 1
TIRF, Structured Illumination
Kenworthy
12:00-1:00 pm Lunch
1:00-4:30 pm LAB
CONFOCAL MICROSCOPY
4:30-5:30 pm Tips and Tricks
Practical Digital Imaging
Watkins
6:00-7:00 pm Dinner
8:00-11:00 pm LAB
IMAGE PROCESSING & FEATURE EXTRACTION
June 15, 2010
9:00-9:30 am Image Processing 1
Digital Filters
Watkins
9:30-10:30 am Image Processing 2
Deconvolution
Piston
10:30-11:00 am Break
11:00-12:00 pm Confocal 3
3D Methods, Microfluorimetry Applications
St. Croix
12:00-1:00 pm Lunch
1:00pm-4:30 pm LAB
MULTICOLOR 3-D IMAGING
4:30-5:30 pm Tips and Tricks
Cell Labeling, Antibodies, GFP, Practical Aspects
Watkins
6:00-7:00 pm Dinner
Evening Off
June 16, 2010
9:00-10:30 am Live Cell Imaging 2
Advanced Imaging Techniques, Quantitation
Kenworthy
10:30-11:00 am Break
11:00 am - 12:00 pm Multiphoton 1
Theory, Instrumentation, Applications
Piston
12:00 noon Course Photo
12:00-1:00 pm Lunch
1:00-4:30 pm LAB
DECONVOLUTION, APOTOME, FRAP
4:30-5:30 pm Tips and Tricks
Live Cell Imaging Microscope Design
Watkins
6:00-7:00 pm Dinner
7:00-8:00 pm LAB
MULTIPHOTON, TIRF LAB
June 17, 2010
9:00-10:30 am Live Cell Imaging 3
Multicolor and Ratio Imaging
St. Croix
10:30-11:00 pm Break
11:00-12:00 pm Live Cell Imaging 4
FRET and Live Cell Imaging
Kenworthy
12:00-1:00 pm Lunch
1:00-4:00 pm LAB
LIVE CELL FRET IMAGING
4:00-4:30 pm LAB
LIVE CELL CHAMBERS, SETUP COLLABORATIVE GROUPS BASED ON STUDENT INTERESTS
4:30-5:30 pm Tips and Tricks
How to Manage Data, Make Movies, Etc
Staff
6:00-7:00 pm Dinner
7:00-11:00 pm LAB
RATIO IMAGING METHODS TRACKING, QUANTITATION, STUDENT EXPERIMENTS
June 18, 2010
9:00-10:00 am Live Cell Imaging 5
Comparison of Optical Sectioning Approaches
Piston
10:00-12:00 am LAB
COLLECT DATA, PREPARE PRESENTATIONS
12:00-2:00 pm Lunch
2:00-4:00 pm Results!
Student Presentations
4:00-4:30 pm Mechanics
How Do I Get Money to Pay for All This Stuff?
Watkins
4:30-5:00 pm Summing up
Q&A, Student Feedback
Faculty
5:30 pm Lobster Bake
Caravan to Thompson Island Picnic Area
 
 
Organized by: Mount Desert Island Biological Laboratory and University of Pittsburgh School of Medicine
Invited Speakers:

Simon C. Watkins, Ph.D., University of Pittsburgh School of Medicine

David W. Piston, Ph.D.,  Vanderbilt Medical Center

Claudette M. St. Croix, Ph.D., University of Pittsburgh Graduate School of Public Health

Anne Kenworthy, Ph.D., Vanderbilt University

 
Deadline for Abstracts: n/a
 
Registration:

Application form is online at the course website.

E-mail: jmedlin@mdibl.org
 
   
 
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