Continuing Professional Development , Oxford
10th -12th December 2009
Day 1: Morning Session - Basic Concepts in Stem Cell Biology Lecture 1 - Stem Cells: Definitions and Principles - Defining features of a stem cell: self-renewal vs asymmetric cell division
- Differentiation and the role of transit amplifying cells
- Transdifferentiation: Fact or Artefact?
- Stem cell quiescence as a counterbalance to exhaustion
Dr Paul Fairchild, Sir William Dunn School of Pathology, University of Oxford Lecture 2 - Pluripotency: Molecular Mechanisms - Definitions: totipotency, pluripotency and multipotency
- The transience of pluripotency during embryogenesis
- Role of transcription factors: Oct4 and Nanog
- Assays of pluripotency and issues for the definition of human ES cells
Prof Elizabeth Robertson, Sir William Dunn School of Pathology, University of Oxford Lecture 3 - The Stem Cell Niche - The dominant nature of the niche and the control of stem cells homeostasis
- The role of intrinsic and extrinsic factors
- The importance of the plane of cell division in self renewal and differentiation
- Examples of the niche: primordial germ cells vs the crypt of Lieberkuhn
Dr Carolyn Carr, Dept of Physiology, Anatomy and Genetics, University of Oxford Lecture 4 - Cancer Stem Cells: A new Paradigm - The concept of the cancer stem cell
- Susceptibility of adult stem cells to transformation
- Evidence of the cancer stem cell from leukaemia, ovarian and prostate cancer
- Novel therapies that target the cancer stem cell
Prof Tariq Enver, Weatherall Institute of Molecular Medicine, University of Oxford Lecture 5 - Dedifferentiation and Induced Pluripotency - Dedifferentiation and Induced Pluripotency
- Dedifferentiation in vitro and in vivo
- Case studies of dedifferentiation: oligodendrocytes vs myocytes
- Induced pluripotency: the role of specific transcription factors
- Demonstration of induced pluripotency in mouse and man
Dr Ashleigh Boyd, Sir William Dunn School of Pathology, University of Oxford Day 1: Afternoon Session - Stem Cell Populations: Adult versus Embryonic Lecture 1 - Haematopoietic Stem Cells - Haematopoietic Stem Cells
- Phenotype and properties of HSC
- The HSC niche: vascular vs osteoblastic
- Differentiation and cell fate determination
- Therapeutic opportunities: hematological malignancies and primary immune deficiencies
Dr Neil Rodrigues, Weatherall Institute for Molecular Medicine, University of Oxford Lecture 2 - Mesenchymal Stem Cells - Isolation, culture and phenotype of MSC
- Properties and function of MSC in vivo
- Immunosuppressive properties of MSC
- Therapeutic opportunities: eg lysosomal storage diseases and Osteogenesis imperfecta
Prof Jim Triffitt, Nuffield Dept of Orthopaedic Surgery, University of Oxford Lecture 3 - Neural Stem Cells - Identification of NSC and their anatomical locations
- Differentiation potential in vitro and in vivo
- Anatomical integration of NSC-derived neurons
- Therapeutic opportunities: Parkinson's disease and spinal cord injury
Dr Francis Szele, Dept of Physiology, Anatomy and Genetics, University of Oxford Lecture 4 - Cardiac Stem Cells - Isolation, culture and characterisation of cardiac stem cells
- Differentiation potential in vitro and in vivo
- Anatomical and electrical integration into cardiac muscle
- Therapeutic opportunities: myocardial infarction
Prof Kieran Clarke, Dept of Physiology, Anatomy and Genetics, University of Oxford Lecture 5 - Embryonic Stem Cells - Derivation of ES cells and their properties
- ES cell culture and the maintenance of pluripotency
- Embryoid body formation during differentiation
- Assays of pluripotency: chimera and teratoma formation
Dr Frances Brook, Nuffield Dept of Obstetrics and Gynaecology, University of Oxford Day 2: Morning Session - Applied Topics in Stem Cell Biology Lecture 1 - Practicalities of Stem Cell Culture - Difficulties of maintaining hESC and the problems of scale-up
- Enzymatic vs non-enzymatic approaches
- Use of the ROCK inhibitor
- Feeder-free cultures
- Assessment of pluripotency by expression of Oct4 etc
Dr Kate Silk, Sir William Dunn School of Pathology, University of Oxford Lecture 2 - Medical Applications of Stem Cell Biology - History of stem cell therapy: the story of bone marrow transplantation
- Use of ES cells for toxicology or as vehicles for gene therapy
- Cell replacement therapy: successes and persistent obstacles
- Induced pluripotency: future prospects for therapy
Prof Len Seymour, Dept of Clinical Pharmacology, University of Oxford Lecture 3 - Cord Blood Stem Cells: Certainties and Controversies - Phenotype and biology of cord blood stem cells
- Controversies surrounding their pluripotency
- Banking of cord blood as a future source of stem cells
- Medical applications of cord blood stem cells
Prof Suzanne Watt, Nuffield Dept of Clinical Laboratory Sciences, University of Oxford Lecture 4 - Taming the Immune System to Tolerate Stem Cell Grafts - The basic immunology of rejection
- Immunogenicity of ES cells and their progeny
- Acquired immune privilege of ES cell-derived tissues
- Strategies for the induction of immunological tolerance
Dr Paul Fairchild, Sir William Dunn School of Pathology, University of Oxford Lecture 5 - Advances in Tissue Engineering - Automation in stem cell culture
- Oxygen tension as an important variable in stem cell culture
- The design of 3-dimensional scaffolds
- Successes in tissue engineering: heart values, ears and bladder
Prof Jan Czernuszka, Dept of Materials, University of Oxford Day 2: Afternoon Session - Regulatory and Ethical Issues Lecture 1 - Legal and Regulatory Aspects of Stem Cell Biology - A survey of legislative frameworks: USA, Germany and South Korea
- Current UK legislation and the role of the HFEA
- Recent parliamentary bills on cybrid technology and saviour siblings
- The role of the National Stem Cell Bank
Dr Glyn Stacey, UK Stem Cell Bank, South Mimms Lecture 2 - The Ethics of Cloning and Stem Cell Research - The problems of media hype and public perception of stem cell biology
- Derivation of ES cells: a necessary evil?
- Therapeutic vs reproductive cloning: where do we draw the line?
- ES cell-derived gametes and the ethics of induced pluripotency
Dr Matthew Liao, Programme on Ethics of the New Biosciences, University of Oxford Lecture 3 - Open Discussion of Ethical Issues Dr Matthew Liao, Programme on Ethics of the New Biosciences, University of Oxford Lecture 4 - Intellectual Property Rights and Patenting Issues - The complexities of patenting in the stem cell arena
- Differences between US, European and Australian perspectives
- Case study: the WARF patents for hES cells
- A survey of current patents in the stem cell field
Dr Lee Chapman, J A Kemp & Co Chartered Patent Attorneys, London Lecture 5 - Commercialisation of Stem Cell Biology - The ageing population and the market for stem cell therapies
- Current obstacles to the clinical implementation of stem cell therapies
- Risk stacking: technological, regulatory and market risks
- Current contenders in the race for therapeutic opportunities
Prof Chris Mason, Regenerative Medicine Bioprocessing Unit, UCL Content subject to change
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