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Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
July 25 - August 14, 2006
Advances in light microscopy, digital image processing, and the development of a variety of powerful fluorescent probes present expanding opportunities for investigating the nervous system, from synaptic spines to networks in the brain. This intensive laboratory and lecture course will provide participants with the theoretical and practical knowledge to utilize emerging imaging technologies. The primary emphasis of the course will be on vital light microscopy. Students will learn the principles of light microscopy, as well as use of different types of electronic cameras, laser scanning systems, functional fluorophores, delivery techniques, and digital image-processing software. In addition to transmitted light microscopy for viewing cellular motility, the course will examine a variety of molecular probes of cell function, including calcium-sensitive dyes, voltage-sensitive dyes, photo-activated ("caged") compounds, and exocytosis tracers. Issues arising in the combination of imaging with electrophysiological methods will be covered. Particular weight will be given to multi-photon laser scanning microscopy and to newly available biological fluorophores, especially Green-Fluorescent Protein (GFP) and its variants. We will use a spectrum of neural and cell biological systems, including living animals, brain slices, and cultured cells. Applicants should have a strong background in the neurosciences or in cell biology.
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Organized by:
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Florian Engert, Mark Hubener, David Kleinfeld, Jack Waters |
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Invited Speakers:
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Guest Lecturers to be announced
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Deadline for Abstracts:
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March 15, 2006 for applications
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Registration:
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Apply here
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E-mail:
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meetings@cshl.edu
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