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HUM-MOLGEN
-> Events
-> Courses and Workshops |
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December 13, 2005 |
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Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
July 25 - August 14, 2006
The Eukaryotic Gene Expression Course is designed for students, postdocs, and principal investigators who have recently ventured into the exciting area of gene regulation. The course will focus on state-of-the-art strategies and techniques employed in the field. Emphasis will be placed both on in vitro and in vivo protein-DNA interactions and on novel methodologies to study gene regulation. Students will make nuclear extracts, perform in vitro transcription reactions and measure RNA levels using primer extension. Characterizations of the DNA-binding properties of site-specific transcription factors will be carried out using electrophoretic mobility shift and DNase I footprinting assays. In addition, students will learn techniques for the assembly and analysis of chromatin in vitro. This will include transcription assays, chromatin footprinting and chromatin remodeling assays. Over the past few years, the gene regulation field has developed in vivo approaches to study gene regulation. Students will be exposed to the chromatin immunoprecipitation technique. They will also use RNAi for specific knock-down experiments in mammalian cells. In addition, determining cellular gene expression profiles has been accelerated tremendously by DNA microarray technology. Students will receive hands-on training in performing and interpreting results from DNA microarrays.
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Organized by:
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Michael Bulger, Thomas Oelgeschlager, Ali Shilatifard, Laszlo Tora |
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Invited Speakers:
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Guest Lecturers to be announced
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Deadline for Abstracts:
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March 15, 2006 for applications
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Registration:
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Apply here
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E-mail:
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meetings@cshl.edu
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