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University of York, York, UK
17-22 April 2005
An intensive, hands-on, five-day training course in the theory and practice of modern proteomics techniques. The course will cover sample preparation, two-dimensional gel electrophoresis, in-gel tryptic digestion, mass spectrometry, and database searching. Participants are encouraged to bring their own samples for analysis.Hands-on laboratory activities will include: * 2D gel electrophoresis
* Gel analysis using the latest software, including Nonlinear Dynamics Phoretix 2D
* In-gel digestion and MALDI sample preparation
* MALDI-MS and MS/MS using an Applied Biosystems 4700 Proteomics Analyzer
* ES-LC-MS/MS using an Applied Biosystems QSTAR®
* Submission of mass spectral data to Mascot database searching
* Interpretation of database search results Classroom sessions will cover the following topics: * Experimental design for proteomics studies
* Protein and peptide sample-handling for proteomics
* 2D Gel electrophoresis
* Mass spectrometry in proteomics
* Interpretation of CID tandem mass spectra of peptides
* Database searching with MS and MS/MS spectral data
* Analysis of post-translational modifications
* Troubleshooting proteomics experiments
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Organized by:
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University of York & Applied Biosystems |
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Invited Speakers:
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Course Tutors: * Professor Jane Thomas-Oates, Department of Chemistry, University of York
* Dr Peter Ashton, Department of Biology, University of York
* Dr Rachel Curwen, Department of Biology, University of York
* Dr Jerry Thomas, Technology Facility, University of York
* Dr Martin Hornshaw, Business Development Scientist - Proteomics, Applied Biosystems
* Dr Alison Ashcroft, Mass Spectrometry Facility Manager, University of Leeds
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Deadline for Abstracts:
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Early registration recommended
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Registration:
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Registration instructions available from our website.
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E-mail:
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baj2@york.ac.uk
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