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Practical Proteomics

  September 23, 2004  

University of York, York, UK
17-22 April 2005

An intensive, hands-on, five-day training course in the theory and practice of modern proteomics techniques. The course will cover sample preparation, two-dimensional gel electrophoresis, in-gel tryptic digestion, mass spectrometry, and database searching. Participants are encouraged to bring their own samples for analysis.

Hands-on laboratory activities will include:

* 2D gel electrophoresis
* Gel analysis using the latest software, including Nonlinear Dynamics Phoretix 2D
* In-gel digestion and MALDI sample preparation
* MALDI-MS and MS/MS using an Applied Biosystems 4700 Proteomics Analyzer
* ES-LC-MS/MS using an Applied Biosystems QSTAR®
* Submission of mass spectral data to Mascot database searching
* Interpretation of database search results

Classroom sessions will cover the following topics:

* Experimental design for proteomics studies
* Protein and peptide sample-handling for proteomics
* 2D Gel electrophoresis
* Mass spectrometry in proteomics
* Interpretation of CID tandem mass spectra of peptides
* Database searching with MS and MS/MS spectral data
* Analysis of post-translational modifications
* Troubleshooting proteomics experiments

Organized by: University of York & Applied Biosystems
Invited Speakers: Course Tutors:

* Professor Jane Thomas-Oates, Department of Chemistry, University of York
* Dr Peter Ashton, Department of Biology, University of York
* Dr Rachel Curwen, Department of Biology, University of York
* Dr Jerry Thomas, Technology Facility, University of York
* Dr Martin Hornshaw, Business Development Scientist - Proteomics, Applied Biosystems
* Dr Alison Ashcroft, Mass Spectrometry Facility Manager, University of Leeds

Deadline for Abstracts: Early registration recommended
Registration: Registration instructions available from our website.
E-mail: baj2@york.ac.uk
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